A Validated RP-HPLC Method Development for Determination of Anthraquinone Marker from the Roots of Rubia cordifolia Linn.

Khodke AS; Potale LV; Patole SM; Damle MC

Research Journal of Pharmacy and Technology

ISSN

0974-360X (Online)
0974-3618 (Print)

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A Validated RP-HPLC Method Development for Determination of Anthraquinone Marker from the Roots of Rubia cordifolia Linn.

Author(s): Khodke AS, Potale LV, Patole SM, Damle MC

Email(s): mcdamle@rediffmail.com

DOI: Not Available

Address: Khodke A.S., Potale L.V., Patole S.M., and Damle M.C.*
A.I.S.S.M.S. College of Pharmacy, Kennedy Road, Near R.T.O., Pune - 411 001.
*Corresponding Author

Published In: Volume - 4, Issue - 2, Year - 2011

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ABSTRACT:
Herbal medicines have good efficacy, safety, and lesser side effects. They are in great in demand in developed world for primary health care. India has rich traditional knowledge, heritage of herbal medicines and large biodiversity but despite it India has dismal share of world market. Many Pharma companies market herbal preparation as neutraceutical and take excuses from quality control parameter set by W.H.O. India has thousands of medicinal plants but in Indian Pharmacopoeia, Quality Control parameter of only 56 plants are included. Rubia cordifolia Linn has good pharmacological activity. Both stem and roots of Rubia cordifolia Linn have pharmacological activity. But in I.P. 2007, marker designation for stem of Rubia cordifolia Linn is included. Hence we have developed and validated HPLC method for marker isolated from roots of Rubia cordifolia Linn. Marker was isolated from roots Rubia cordifolia Linn and purified. RP- HPLC method for marker was developed using mobile phase Methanol: water in the ratio of 80:20. Stationary phase used for this method was Hi-Qsil C18 (250 × 4.6mm i.d.). Using this mobile phase marker gets eluted at RT 4.294. This RP-HPLC method was validated as per ICH guideline.

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Cite this article:
Khodke AS, Potale LV, Patole SM, Damle MC. A Validated RP-HPLC Method Development for Determination of Anthraquinone Marker from the Roots of Rubia cordifolia Linn. Research J. Pharm. and Tech. 4(2): February 2011; Page 326-328.

Cite(Electronic):
Khodke AS, Potale LV, Patole SM, Damle MC. A Validated RP-HPLC Method Development for Determination of Anthraquinone Marker from the Roots of Rubia cordifolia Linn. Research J. Pharm. and Tech. 4(2): February 2011; Page 326-328. Available on: https://rjptonline.org/AbstractView.aspx?PID=2011-4-2-25