M. Yasmin Begum, Mahammed Rafi Shaik, K. Abbulu, M. Sudhakar
M. Yasmin Begum1*, Mahammed Rafi Shaik1, K. Abbulu2 and M. Sudhakar1
1Malla Reddy College of Pharmacy, Maisammaguda, Secunderabad.
2Malla Reddy Institute of Pharmaceutical Sciences, Maisammaguda, Secunderabad.
Volume - 4,
Issue - 11,
Year - 2011
The objective of the present study was to prepare ketorolac loaded conventional liposomes containing natural phospholipid, Soy phosphatidyl choline by optimizing various process and formulation related variables such as drug - lipid ratio, cholesterol content, vacuum, speed of rotation, hydration medium and hydration time. Effects of charges over the vesicles were studied by incorporating dicetylphosphate and stearylamine. Various liposomal batches were prepared by thin film hydration technique and they were characterterized for drug encapsulation %, chemical interaction of the components, vesicle size profile, drug release profile in vitro and stability using specified methods. The best ketorolac loaded liposome was composed of SPC/Cholesterol/stearyl amine group of 4:1:0.7%w/w/w. It was found to have greater stability, higher drug encapsulation % and lower drug release in vitro compared to the others. The drug encapsulation efficiency of optimum formulation was 84% and it could retain 36% of the drug even after 24h. FTIR study indicated that there is no significant chemical interaction between the components. SEM photograph confirmed that vesicles were homogenous and spherical in shape. Stability studies showed that the vesicles were stable in refrigerated temperature for nearly one month without significant difference in drug entrapment. The present study has given us knowledge also that liposomes with low amount of cholesterol are better candidate for ketorolac liposomes.
Cite this article:
M. Yasmin Begum, Mahammed Rafi Shaik, K. Abbulu, M. Sudhakar. Ketorolac Tromethamine Loaded Liposomes: Development, Characterization and In Vitro Evaluation. Research J. Pharm. and Tech. 4(11): Nov. 2011; Page 1766-1771.