Tannins are the complex secondary metabolites have been used since earlier period as tanning agents and reported for astringent, anti-inflammatory, antidiarrhoeal and antimicrobial activities. Because of their chemical complexity and difficulty in isolation due to their close association with flavnoids, it is necessary to estimate the total tannins. Also it has been observed that production of the tannins in different situations and climatic conditions may vary. In the present work we have tried to check any seasonal variation in tannin content of different parts of Memecylon umbellatum. In the present work we have selected leaves, stem bark, stem wood, root and inflorescences for seasonal variation in tannin content. All the parts except inflorescences were collected in summer, winter and rainy season. Tannin estimation was determined by U.V. spectrophotometer using Folin-Denis method. Calibration curve was obtained using standard Gallic acid and tannin content was estimated using fine powder of all parts of the plant with and without acid hydrolysis. Calibration curve shows coefficient of variance (r2) = 0.9998 and tannin content was found to be highest in bark drug (2.4125 ± 0.124) collected in summer compare to winter and rainy seasons. Tannin content was found decreased in leaves, roots and Stem wood respectively. Inflorescence showed minimum amount of tannins (0.08639 ± 0.064). Also variation of tannins from summer to rainy season was observed in all the tested parts and found more in root (Avg. 0.28%) and minimum in leaves (Avg. 0.034%). Variation was also observed when tested for consecutive years at different seasons.
Cite this article:
Killedar SG, More HN. Seasonal Variation of Tannin Content in Different Parts of Memecylon umbellatum Burm. Research J. Pharm. and Tech. 4 (1): January 2011; Page 78-81.