Author(s): P Venkata Suresh, GV Srujana, G Lavanya, KML Manoja, M Hadassah, B Srilekha, S Vasudha Lakshmi and N Rama Rao

Email(s): sureshpharma78@gmail.com

DOI: Not Available

Address: P. VenkataSuresh*, G.V. Srujana, G. Lavanya, K.M.L. Manoja, M. Hadassah, B. Srilekha, S. Vasudha Lakshmi and N.Rama rao
Dept. of Pharmaceutical Analysis, Chalapathi Institute of Pharmaceutical Sciences, Lam, Guntur-34, India.
*Corresponding Author

Published In:   Volume - 4,      Issue - 1,     Year - 2011


ABSTRACT:
A simple, sensitive, rapid, robust and reproducible method for the determination of Raloxifene hydrochloride in bulk and pharmaceutical formulation (Tablets) was developed using reverse phase high performance liquid chromatographic method (RP-HPLC). The RP-HPLC analysis was performed isocratically on Equisil-ODS (octadecylsilane; C18), 5µ, 4.6 x 250 mm analytical column using a mobile phase consisting of 0.02M potassium dihydrogen ortho Phosphate buffer (pH adjusted to 3.00 ± 0.01 with ortho phosphoric acid after addition of 0.1% triethyl amine), Methanol, Acetonitrile in the Ratio of 40:30:30 (v/v/v) with a flow rate of 1mL/min. The analyte was monitored with UV detector at 282 nm. The developed method Raloxifenehydrochloride elutes at a retention time of 2.70 min. The proposed method is having linearity in the concentration range from10 to70 µg/mL of Raloxifenehydrochloride. The present method was validated with respect to system suitability, linearity, precision, limit of detection (LOD) and limit of quantification (LOQ), accuracy (recovery), ruggedness, robustness, stability, forced degradation studies (specificity).The proposed method can be readily utilized for bulk drug and pharmaceutical formulations.


Cite this article:
P Venkata Suresh, GV Srujana, G Lavanya, KML Manoja, M Hadassah, B Srilekha, S Vasudha Lakshmi and N Rama Rao. Development and Validation of Isocratic RP-HPLC Method for Raloxifene Hydrochloride in Bulk and Pharmaceutical Formulation. Research J. Pharm. and Tech. 4 (1): January 2011; Page 146-149.


Recomonded Articles:

Author(s): Nimbalkar V.V., Pansare P.M., Nishane B.B.

DOI: 10.5958/0974-360X.2015.00310.8         Access: Open Access Read More

Author(s): Umesh Kumar, Rakesh Narang, Surendra Kumar Nayak, Sachin Kumar Singh, Vivek Gupta

DOI: 10.5958/0974-360X.2017.00425.5         Access: Open Access Read More

Author(s): Amurdhavani. B.S

DOI: 10.5958/0974-360X.2016.00299.7         Access: Closed Access Read More

Author(s): Ravindranath S. Misal, Vishawas R. Potphode, Vijay R. Mahajan

DOI: 10.5958/0974-360X.2017.00218.9         Access: Open Access Read More

Author(s): Niharika, Navneet Verma

DOI: 10.5958/0974-360X.2016.00182.7         Access: Open Access Read More

Author(s): Rakhi Mehra, Renu Makhija, Neera Vyas

DOI: Not Available         Access: Open Access Read More

Author(s): A. S. K. Sankar, B. Datchayani, N. Balakumaran, Mohammed Rilwan, R. Subaranjani

DOI: 10.5958/0974-360X.2017.00047.6         Access: Open Access Read More

Author(s): Jino Elsa Thomas, Usha Y Nayak*, Jagadish PC, Koteshwara KB

DOI: 10.5958/0974-360X.2017.00007.5         Access: Open Access Read More

Author(s): R. Narayana Charyulu, P. Parvathy Devi, Jobin Jose, A. Veena Shetty

DOI: Not Available         Access: Open Access Read More

Author(s): G. Bhavani, R. Navaneethan

DOI: 10.5958/0974-360X.2017.00269.4         Access: Open Access Read More

Author(s): Karishma Ravinthar, Jayalakshmi

DOI: 10.5958/0974-360X.2018.00148.8         Access: Open Access Read More

Author(s): Amina Mehrin Bano, Vishnupriya. V, Gayathri. R

DOI: 10.5958/0974-360X.2016.00171.2         Access: Open Access Read More

Author(s): P.Maheshwari, D.Pavithra, Neethu.T.T, T.S. Shanmugarajan, P. Shanmugasundaram

DOI: 10.5958/0974-360X.2017.00011.7         Access: Open Access Read More

Author(s): Bhawna Goyal, Sunil Agrawal, B.S. Sohi, Ayush Dogra

DOI: 10.5958/0974-360X.2016.00176.1         Access: Open Access Read More

Author(s): Aadil Ahamed.S, Dhanraj. M

DOI: 10.5958/0974-360X.2017.00169.X         Access: Open Access Read More

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

0.38
2018CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags