ABSTRACT:
The work presented here deals with formulation, evaluation and validation of liquid oral preparation of Gymnema sylvestre and Stevia rebaudiana and their combination to study its antidiabetic activity. The antihyperglycemic activity of aqueous extract of Gymnema sylvestre and their combination were evaluated; diabetes was induced in albino rats of either sex by a single intraperitoneal injection of aqueous alloxan monohydrate (120 mg/kg). Single dose study with aqueous extract of Gymnema sylvestre (100 mg/kg) - Combination treated, equal proportion, 100 ml/kg and showed significant (p < 0.001) decrease in serum glucose level at 2, 4 and 6 hr. Continuous treatment with aqueous extract of Gymnema sylvestre (100 mg/kg), Combination treated, equal proportion, 100 ml/kg for a period of 28 days showed a significant decrease (p < 0.001) in serum glucose level in diabetic rats. Maximum reduction of serum glucose level occurred - Combination treated, equal proportion, 100 ml/kg. It may be said that - combination treated, equal proportion, 100 ml/kg of the aqueous extract of Gymnema sylvestre and Stevia rebaudiana decreased the serum glucose level and improved glucose tolerance.