Srivastava Bimal Kumar, Kushwah Dharmendra Kumar, Kolhe Prakash Yashwant, Patel Nitin
Srivastava Bimal Kumar*, Kushwah Dharmendra Kumar, Kolhe Prakash Yashwant and Patel Nitin
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Volume - 3,
Issue - 4,
Year - 2010
This paper describes a validated method for the quantification of aminoguanidine in drug substances and their intermediates using High Performance Liquid Chromatography (HPLC), with a per column derivatization with 1-Naphthyl isothiocyanate. Derivative formed was analysed using a RP-18 HPLC column with pH 3 ortho-phosphoric acid and triethyleamine buffer and Methanol as mobile phase with a gradient elution. Wavelength 220 nm was used for detection. The method is validated for its Specificity, Precision, Accuracy, Linearity Ruggedness and Robustness. Aminoguanidine is linear from 0.015µg/ml to 0.750 µg/ml with correlation coefficient 0.99609. Limit of Quantitation of the method is 0.015 µg/ml and Limit of Detection is 0.010 µg/ml.
Cite this article:
Srivastava Bimal Kumar, Kushwah Dharmendra Kumar, Kolhe Prakash Yashwant, Patel Nitin. Validated HPLC Method for Determination of Contents of Residual Aminoguanidine in Drug Substances. Research J. Pharm. and Tech.3 (4): Oct.-Dec.2010; Page 1058-1060.