Author(s): M Yashpal Naidu, K P Channa Basavaraj, T Tamizh Mani, K Roopa

Email(s): yashpal.rasky@gmail.com

DOI: Not Available

Address: M. Yashpal Naidu*, K. P. Channa Basavaraj, T. Tamizh Mani and K. Roopa
Department of Pharmaceutical Analysis, Bharathi College of Pharmacy, Bharathi Nagara, Mandya District, Karnataka, India - 571422.
*Corresponding Author

Published In:   Volume - 3,      Issue - 3,     Year - 2010


ABSTRACT:
A simple, specific, accurate, precise and sensitive Reverse Phase - High Performance Liquid Chromatographic method has been developed for the quantitation of Pioglitazone in both Bulk drug and Pharmaceutical dosage forms. A Venusil XBP C18, 5 µm column having 250 × 4.6 mm internal diameter in isocratic mode with mobile phase containing Phosphate buffer - Acetonitrile (50: 50). The flow rate was 1.0 ml / min and the effluents were monitored at 225 nm. The retention time was 5.93 min. The linearity was in the range of 25 - 75 µg / ml. This method was validated for linearity, precision, limit of detection, limit of quantitation, accuracy, ruggedness and robustness. Statistical analysis proves that the method is reproducible and selective for the estimation of the said drug.


Cite this article:
M Yashpal Naidu, K P Channa Basavaraj, T Tamizh Mani, K Roopa. Validated RP-HPLC Method for the Quantitation of Pioglitazone an Anti - Diabetic Drug in Bulk and Pharmaceutical Dosage Forms. Research J. Pharm. and Tech.3 (3): July-Sept. 2010; Page 885-887.


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DOI: 10.5958/0974-360X 

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