A simple, specific, accurate, isocratic and stability indicating RP-HPLC method was developed for determination of famciclovir in bulk drug and pharmaceuticals. The best separation of famciclovir and its degradation products was achieved on reversed phase C18 column with dimensions of 250 mm × 4.6 mm i. d., 5-µm particle size. The mobile phase was composed of methanol: KH2PO4 buffer (pH 3.0, with ortho phosphoric acid) (35:65, v/v) with flow rate 1.0 mL/min. The separation was performed at ambient temperature and eluents were monitored at 242 nm. The proposed method was statistically validated for linearity, accuracy, precision and specificity. The linearity of famciclovir peak area responses was demonstrated within range of 10-50 µg mL-1 (r =0.9997). Famciclovir was kept under the different stress conditions like acid, neutral, alkaline, oxidative, and photolytic. Employing RP-HPLC method, separation of drug peak from the degradation product peaks and no interference from tablet excipients in assay method shows the proposed method is stability indicating one.
Cite this article:
DD Patil, RA Fursule. A Stability Indicating LC Method for Famciclovir. Research J. Pharm. and Tech.3 (3): July-Sept. 2010; Page 762-765.