Author(s): Rupali Kirtawade, Pallavi Salve, Anita Kulkarni, Pandurang Dhabale

Email(s): rupalikalp123@rediffmail.com

DOI: Not Available

Address: Rupali Kirtawade*, Pallavi Salve, Anita Kulkarni and Pandurang Dhabale
Govt. College of Pharmacy, Karad.
*Corresponding Author

Published In:   Volume - 3,      Issue - 3,     Year - 2010


ABSTRACT:
Hydrophilic Interaction Liquid Chromatography (HILIC) is a variant of normal phase chromatography that performed with a very polar stationary phase and a mostly organic mobile phase. When the mobile phase contains > 60% organic solvent, then hydrophilic interaction becomes significant. The mechanism of HILIC implicates partitioning between a water-enriched layer partially immobilized on the hydrophilic stationary phase and the less polar mobile phase. A chromatographic system for HILIC chromatography is essentially instrumental identical to RPLC (Reversed Phase Liquid Chromatography) systems. HILIC columns contain a stationary phase that is hydrophilic and quite often also charged, at least in some region of the pH-scale. Compounds separated on the column interact with the stationary phase and are generally more strongly retained the more hydrophilic the compound. It is used for determination of dimethindene maleate in topical gel, zanamivir in rat and monkey plasma, tromethamine as the counter ion in an investigational pharmaceutical salt.


Cite this article:
Rupali Kirtawade, Pallavi Salve, Anita Kulkarni, Pandurang Dhabale. Hydrophilic Interaction Liquid Chromatography (HILIC). Research J. Pharm. and Tech.3 (3): July-Sept. 2010; Page 640-643.


Recomonded Articles:

Author(s): Ravindranath S. Misal, Vishawas R. Potphode, Vijay R. Mahajan

DOI: 10.5958/0974-360X.2017.00218.9         Access: Open Access Read More

Author(s): Niharika, Navneet Verma

DOI: 10.5958/0974-360X.2016.00182.7         Access: Open Access Read More

Author(s): A. S. K. Sankar, B. Datchayani, N. Balakumaran, Mohammed Rilwan, R. Subaranjani

DOI: 10.5958/0974-360X.2017.00047.6         Access: Open Access Read More

Author(s): U.S Mahadeva Rao, Khamsah Suryati Mohd, Abdurrazaq Muhammad, Bashir Ado Ahmad, Mohaslinda Mohamad, Rosmawati Mat Ali

DOI: Not Available         Access: Open Access Read More

Author(s): Rekha Rajendran, R Hemachander, T Ezhilarasan, C Keerthana, DL Saroja, KV Saichand, Mohamed Gasim Abdullah

DOI: Not Available         Access: Open Access Read More

Author(s): Vandana Gautam, Dhriti Kapoor, Saroj Arora, Renu Bhardwaj*

DOI: 10.5958/0974-360X.2016.00166.9         Access: Open Access Read More

Author(s): Archana Kushwaha, Jayanti Jaiswal, Priya Singh, Neha Rathore, Jai Prakash Dhruw, Ishu Sahu, Chhaya Singh, Krinsha Kumar Sahu, Mukta Agrawal, D. K. Tripathi, Ajazuddin, Amit Alexander

DOI: 10.5958/0974-360X.2017.00270.0         Access: Open Access Read More

Author(s): Mukta D. Naykode, Durgacharan A. Bhagwat, Swapnil D. Jadhav, Harinath N. More

DOI: 10.5958/0974-360X.2017.00133.0         Access: Open Access Read More

Author(s): Mayanka Singh, Manoj Charde, Rajesh Shukla, Rita M. Charde

DOI: Not Available         Access: Open Access Read More

Author(s): Narendra Chotai, Vishnu Patel, Harsha Patel, Uren Patel, Rajendra Kotadiya

DOI:         Access: Open Access Read More

Author(s): Homendra Kumar Sahu, Kamdev Sen, Raju Patel, Hulash Sahu, Deepika Sahu, Gulshan Sahu, Chandrashekhar Jain, Narayan Prasad Chandra, Mukta Agrawal, D. K. Tripathi, Ajazuddin, Amit Alexander

DOI: 10.5958/0974-360X.2017.00173.1         Access: Open Access Read More

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

0.38
2018CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags