Author(s): Jitendra Verma, Dheeraj Jain, Nilesh Jain, Sharad P Pandey, Deepak Kumar Jain


DOI: Not Available

Address: Jitendra Verma1, Dheeraj Jain1, Nilesh Jain2, Sharad P Pandey3 and Deepak Kumar Jain3*
1 Shri Gujrati Samaj College of Pharmacy, Indore (M.P.) - 452010, India
2Sagar Institute of Research and Technology-Pharmacy, Bhopal, (M.P), India
3Truba Institute of Pharmacy, Bhopal (M.P.)-462036, India
*Corresponding Author

Published In:   Volume - 3,      Issue - 2,     Year - 2010

A simple, precise, reliable, rapid and reproducible reversed-phase high performance liquid chromatography (RP-HPLC) method was developed and validated for the simultaneous estimation of lamivudine (3TC), stavudine (D4T) and nevirapine (NVP) present in multicomponent dosage forms. Gradient chromatography using Inertsil-ODS-3 (C-18) Column (5 µm, 250mm x 4.60mm) eluted with two mobile phase components: mobile phase A comprising of 0.1% OPA and mobile phase B (methanol) with a flow rate of 1.0 mL/min and a detection wavelength at 254nm. Parameters such as linearity, precision, accuracy, recovery, specificity and ruggedness are studied as reported in the International Conference on Harmonization guidelines. The retention times for lamivudine, stavudine and nevirapine were 2.6, 4.08, and 10.92 min respectively. The calibration curves were linear (r2 > 0.9997 for all three compounds). The linearity range and percentage recoveries for 3TC, D4T and NVP are 30-150, 6-30, 40-200µgml-1and 100.95, 107.46, 106.74% respectively. The correlation coefficients for all components are close to 1. The relative standard deviations for three replicate measurements in three concentrations of samples in tablets are always less than 2%.

Cite this article:
Jitendra Verma, Dheeraj Jain, Nilesh Jain, Sharad P Pandey, Deepak Kumar Jain. Simultaneous Estimation of Lamivudine, Stavudine and Nevirapine by RP-HPLC in Tablet Formulation. Research J. Pharm. and Tech. 3(2): April- June 2010; Page 490-493.

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RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

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