A simple, rapid and economical HPLC method and two colorimetric methods have been developed for the estimation of cefepime in its bulk and Pharmaceutical dosage forms. Method I involved an isocratic elution of Cefepime in a column of Phenomenex C18, 250 X 4.6, 5µ, using a mobile phase composition of Methanol: Water (70:30, v/v). The flow rate was 0.8 ml/min and the analyte was monitored at 250 nm. Method II was based on oxidative coupling reaction between cefepime and MBTH in presence of ferric chloride anhydrous to produce a green colored chromogen with an absorption maximum of 639nm. The chromogen obeyed linearity in the range 2-12 µg/ml. Method III is based on reaction between cefepime and 1, 10 phenanthroline in presence of ferric ammonium sulphate to produce a red color chromogen with absorption maximum of 493nm. The chromogen obeyed linearity in the range 0.5-3µg/ml.
Cite this article:
Jane J, Prashant Kumar D, EVS Subrahmanyam. HPLC and Colorimetric Methods for Estimation of Cefepime. Research J. Pharm. and Tech. 3(1): Jan.-Mar. 2010; Page 102-105.