V Kiran Kumar, N Appala Raju, Shabana Begum, Jvln Seshagiri Rao, T Satyanarayana
V Kiran Kumar, N Appala Raju*, Shabana Begum*, Jvln Seshagiri Rao and T Satyanarayana
Department of Pharmaceutical Chemistry, Sree Chaitanya Institute of Pharmaceutical Sciences, LMD.Colony, Karimnagar-505527.AP.
*Department of Pharmaceutical Chemistry, Sultan-Ul-Uloom College of Pharmacy, Mount Pleasant, Road# 3, Banjara Hills, Hyderabad-500 034.
#University College of Pharmaceutical Sciences, Andhra University, Visakhapatnam,AP.
Volume - 2,
Issue - 2,
Year - 2009
A simple, precise, rapid and accurate reverse phase HPLC method developed for the estimation of Gefitinib in tablet dosage form. An Hypersil BDS RP C18, 250x4.6 mm, 5 µm partical size, with mobile phase consisting of 0.02 M Dipotassium Hydrogen ortho phosphate and Methanol in the ratio of 10:90 v/v was used. The flow rate was 1.0 ml/min and the effluents were monitored at 246 nm. The retention time was 3.7 min. The detector response was linear in the concentration of 25-300µg/ml. The respective linear regression equation being Y= 94342.26x+77672.7. The limit of detection and limit of quantification was 0.125µg/ml and 0.15µg/ml respectively. The percentage assay of Gefitinib was 99.5 %. The method was validated by determining its accuracy, precision and system suitability. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of Gefitinib in bulk drug and in its pharmaceutical dosage form.
Cite this article:
V Kiran Kumar, N Appala Raju, Shabana Begum, Jvln Seshagiri Rao, T Satyanarayana. The Estimation of Gefitinib in Tablet Dosage Forms by RP-HPLC. Research J. Pharm. and Tech.2 (2): April.-June.2009; Page 341-343.