SM Sivakumar, L Nirmala, R Swarnalakshmi, L Sivakumar, B Anilbabu, TS Shanmugarajan, J Anbu, V Ravichandran, N Sukumaran
SM Sivakumar1*, L Nirmala2, R Swarnalakshmi2, L Sivakumar2, B Anilbabu2 , TS Shanmugarajan2, J Anbu2, V Ravichandran2 and N Sukumaran3
1Divison of Research and Development, Nehru College of Pharmacy, Pampady, Thiruvilwamala – 680597, Kerala
2Department of Pharmaceutical Biotechnology, VEL’s College of Pharmacy, Pallavaram, Chennai
3Sri Paramakalyani Centre for Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi - 627412
Volume - 2,
Issue - 1,
Year - 2009
Adjuvant aimed to increase the immunogenicity of vaccines remain a focus in vaccine development. The purpose of this research work was to develop an alternative adjuvant for hepatitis B vaccine (HBsAg) to elicit a long lasting immune response after a single dose. The HBsAg encapsulated chitosan microparticles were prepared by emulsion cross linking technique. The newly developed microparticles were evaluated for vaccine content, particle size distribution and in vitro release study. The morphological characters were examined using scanning electron microscope (SEM). The release profile was investigated by the measurement of antigen in the releasing medium at various intervals. The microparticles were release antigen about 6 weeks in vitro. The results indicated that the chitosan polymeric microparticles showing sustained release of HBsAg with an initial burst effect.
Cite this article:
SM Sivakumar, L Nirmala, R Swarnalakshmi, L Sivakumar, B Anilbabu, TS Shanmugarajan, J Anbu, V Ravichandran, N Sukumaran. Preparation and In vitro Evaluation of Hepatitis B Vaccine Encapsulated Chitosan Microparticles. Research J. Pharm. and Tech. 2(1): Jan.-Mar. 2009; Page 117-119.