ABSTRACT:
Quantitative estimation of poorly water-soluble drugs involves use of organic solvents. Major drawbacks of organic solvents include high cost, volatiliy and toxicity. In the present investigation, hydrotropic solubilization is employed to enhance the aqueous solubilities of poorly water-soluble drugs metronidazole and norfloxacin in two-component tablet formulation for simultaneous spectrophotometric determination. Three simple, accurate and economical procedures employed are simultaneous equation method, absorbance ratio method, and dual wavelength method. All methods utilize 8.0M-urea solution as, hydrotropic solubilizing agent. In the urea solution, metronidazole and norfloxacin show maximum absorbance at a wavelength of about 320 and 275 nm respectively and isobestic point is observed at 294nm. The hydrotropic agent and additives used in the manufacture of tablets did not interfere in the analysis. The results of analysis have been validated statistically and by recovery studies.