New Designing and using Electromagnetic Field Device for drawing bacterial growth map
Yousor Majid Jameel1, Dr. Ali Abdul Hussein Mahdi2, Dr. Nihad Khalawe Tektook3*
1Lecturer, Medical Technical Institute - Mansour
2,3Assistant Professor, Middle Technical University-College of Medical and Health Technology- Baghdad - Iraq.
*Corresponding Author E-mail: drnihadkhalawe@gmail.com
ABSTRACT:
In this study, electromagnetic field was used through a device made specifically for this purpose, and several types of bacteria were cultured in a separated manner using culture media used and incubated for 24 hours. With the exposure to the electromagnetic field from the device, the results showed that different bacteria grow in a different pattern, which demonstrate a different map of bacterial growth specific to each type of bacteria. This new technique is very important in the diagnosis of bacteria and determining their types through bacterial growth map after being effected by the electromagnetic field of the device. This finding opens a new field for research in order to discover other properties of the huge numbers of bacteria and to discover the fingerprint for every type of bacteria through this invention, which can make the diagnosis process of bacteria easier.
KEYWORDS: New Designing, Electromagnetic Field Device and bacterial growth map.
INTRODUCTION:
The effects of electromagnetic fields on living organisms have not received the sufficient attention medically, scientifically and socially, except relatively small part. This subject has been left away from peoples' culture and discussion except a fraction of the subject being discussed by people about these effects. Thus, the investigation about the beneficial and harmful effects electromagnetic field is a vital human endeavor. This is because of the organic and psychological effects on humans and other living organisms and in every way of life.
If we examine the electrical and magnetic source, it will appear clearly that it is originally present in the atom of the material. The atom is the fundamental unit of all living components, which will form simple and complex chemical structure, then these complicated structures will form the cells and these cells will form different tissues, which from it the organs was originated, leading to formation of body system, (e.g., respiratory system and GIT system…etc).
So all what we have in our body (and in all living organisms), contain electromagnetic energy, and this energy effect and affect from the surrounded electric and magnetism field (1)
In recent time, the previous art is that there has been a plenty of events concerned with electromagnetic field and it is applications in different scientific fields like physics, engineering and chemistry as well as others. Nonetheless, there have been merely minor steps in studying the effect of electromagnetic field on the growth of microorganisms like bacteria, parasites, fungi and others. Therefore, a need for such studies exists and it was due good fortune that diversity in responses to electromagnetic field was found, which would be mentioned later.
Several scientists have found that bacterial division is affected by electromagnetic field, however, they didn’t noticed the impact on bacterial cultured colonies (3).
MATERIALS AND METHODS:
1. Copper wire 0.5 Mm.
2. Plastic cylinder with diameter of 10 cm.
3. Control panel.
4. Transformer of voltage from 5 volt to 100 volt.
5. Transformer of ampere from 0.5 ampere to 20 ampere.
6. Culture media (nutrient agar).
7. Glass flask.
8. Culture plates.
9. Different types of gram positive and gram negative bacteria.
10. Incubator.
The concept of this new invention is to design a device that is made of two parts; the first part is a hollow coil consist of 1500 (one thousand and five hundreds coils of copper wire) with diameter of 0.5 Mm and the cavity of coil which is in circle shape with diameter 10 cm, connected directly to the second part of the device. The second part consists of control unit running of the system and controlling the voltage and current going through the coil. Setting the required voltage and current depends on cultured bacteria. The control unit consists of voltage transformer coil, current transformer coil and a screen reading the voltage. The device is illustrated in figure (1).
Figure (1): New Designing and using Electromagnetic Field Device for drawing bacterial growth map
Methods:
1 Culture media was prepared and poured in a rectangle sterile container designed specifically for the system. Time was given to the culture media to harden inside the container.
2 E.coli, Klebsiella spp. and Staphylococcus aureus were cultured separately in 3 culturing containers. Culturing process was in a straight line on the surface of culturing media. The containers were put inside the system with the exertion of voltage of 100 volts and current of 20 amperes. The entire system was incubated at 37ͦ C for 24hours.
3 The appearance of cultured bacteria was registered for each bacterial type. Process was repeated for validation of results.
4 The process was repeated without culturing to have a control sample.
RESULTS AND DISCUSSION:
The results showed the growth of each type of bacteria in a distinctive pattern compared to each other as shown in figure (1). This distinctive pattern can be identified as a fingerprint specific to each bacteria, which does not resemble other bacteria.
The implications of this invention include:
1. Diagnosis of bacteria depending on new characteristics which were not assessed before.
2. Opening new horizons for scientific research to explore the effect of electromagnetic fields on bacterial growth.
3. Helps in understanding the beneficial and harmful effects of electromagnetic fields on treatment of bacterial growth inside the human body, which for sure require further and deeper researches.
The advantages of this invention is:
1. A novel concept, which was not tested before.
2. Simple to use and applicable for the study of majority of bacteria and parasites.
3. Add a substantial value for the identification of bacteria and the diagnosis of bacterial types that are difficult to be diagnosed on culture media.
4. Shorten the time required for diagnosis process, especially for types of bacteria that required several tests for diagnosis.
5. In the same manner, it can reduce the cost of diagnosis due the exclusion of some of the expensive testes in the process of diagnosis of bacteria.
The Claims (Elements of Patent):
1 Claim number 1, designing and using Electromagnetic Field Device for drawing bacterial growth map.
2 With reference to claim number 1, the device consists of two parts: the first part is made of a hollow copper coil consists from 1500 (one thousand and five hundreds coils of copper wire) with a diameter of 0.5 mm and the cavity of coil is in circle shape with diameter of 10 cm. Inside this cavity the culture media will be placed. While the second part consists of control unit controlling the voltage and current going to the first part of the device.
3 With the reference to claim number 1, it was possible to draw the map of the appearance of growth of three types of bacteria; E.coli, Klebsiella and staphylococcus aureus. In addition, through this innovation, the growth map for each of these bacteria was determined to be used in bacterial diagnosis.
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Received on 04.03.2019 Modified on 11.04.2019
Accepted on 30.04.2019 © RJPT All right reserved
Research J. Pharm. and Tech 2019; 12(9):4493-4495.
DOI: 10.5958/0974-360X.2019.00774.1