2nd International Conference on Fostering Interdisciplinary Research In Health Sciences (ICFIRHS) 2019 (01-May-2019)        |

Journal :   Research Journal of Pharmacy and Technology

Volume No. :   12

Issue No. :  9

Year :  2019

Pages :   4090-4094

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Detection of HSV and CMV in Pregnant and Miscarriage women by ELISA and real time PCR Assay



Address:   Mariam Kareem Ali1, Jaafar Sataar Shia2, Huda Dhahar Al- marsome3
1Department of Microbiology, College of Medicine – University of Baghdad, Iraq
2Ministry of Health, Baghdad, Iraq
3Department of Microbiology, College of Medicine Al-Nahrain University
*Corresponding Author
DOI No: 10.5958/0974-360X.2019.00704.2

ABSTRACT:
Herpes Simplex Viruses (HSV) and Human Cytomegaloviruses (HCMV) are the major cause of serious viral complications in pregnant women. Screening methods that is ELISA for detecting Herpes Simplex virus(HSV) and Human Cytomegalovirus(HCMV) tend to be slow and insensitive. Therefore in this work, a rapid Real Time PCR (qT-PCR) and ELISA based assay was designed to detect HSV and CMV among pregnant women in Iraq from serum samples. Our study revealed that among the ELISA screened 420 sera samples, 81 (19.3%) of CMV and 17(4.05 %) of HSV were positive, while 64 (12.9%) had positive results for CMV DNA and 5(1.2%) had HSV DNA through real-time PCR. Real time PCR was more sensitive and reliable method in diagnosis of CMV and HSV infections in pregnant women in this comparative study.
KEYWORDS:
HSV, CMV, Miscarriage women, ELISA, PCR.
Cite:
Mariam Kareem Ali, Jaafar Sataar Shia, Huda Dhahar Al- marsome. Detection of HSV and CMV in Pregnant and Miscarriage women by ELISA and real time PCR Assay. Research J. Pharm. and Tech 2019; 12(9):4090-4094.
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