2nd International Conference on Fostering Interdisciplinary Research In Health Sciences (ICFIRHS) 2019 (01-May-2019)        |

Journal :   Research Journal of Pharmacy and Technology

Volume No. :   12

Issue No. :  9

Year :  2019

Pages :   4263-4268

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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5-hydroxymethylfurfural Levels in Coffee and Study of some effecting factors



Address:   Sally Alsubot1, Dima Aldiab2
1Master Student in Analytical and Food Chemistry Department, Faculty of Pharmacy, Tishreen University, Lattakia, Syria.
2Doctor in Analytical and Food Chemistry Department, Faculty of Pharmacy, Tishreen University, Lattakia, Syria.
*Corresponding Author
DOI No: 10.5958/0974-360X.2019.00733.9

ABSTRACT:
5-hydroxymethylfurfural (HMF) is formed during heat processing and\or storage of food. HMF is cytotoxic at high concentrations and could be metabolised to 5-sulfooxymethylfurfural making HMF potentially harmful in an extent unknown at present. Coffee is one of the most important sources of HMF. The aim of this study was to determine HMF in roasted ground coffee and instant coffee consumed in Syria, additionally to study the effect of brewing process, sugar addition and storage on HMF levels. Samples were analyzed by reversed-phase high-performance liquid chromatography RP-HPLC connected to UV detector. The concentrations were 26.16, 76.93, 174.93 mg/kg for hard, moderate and light roasted coffee, respectively, and between 526.56 – 1800.9 mg/kg in instant coffee. HMF contents in instant coffee were significantly higher than ground coffee. HMF content increased about 9.6% by increasing boiling time and 15.66% by heating the mixture of water and coffee until boiling. Additionally, HMF levels increased about 10.84%, 46.99%, 60.24% by the addition of 0.05, 0.1 and 0.3g sugar respectively, where the highest HMF content was formed by the highest sugar addition. HMF content increased about 36% during the first two hours of storage in heat isolator bottle, while remained almost stable in the second ten hours of storage.
KEYWORDS:
5-hydroxymethylfurfural, brewing process, coffee, HPLC, sugar addition.
Cite:
Sally Alsubot, Dima Aldiab. 5-hydroxymethylfurfural Levels in Coffee and Study of some effecting factors. Research J. Pharm. and Tech 2019; 12(9):4263-4268.
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