2nd International Conference on Fostering Interdisciplinary Research In Health Sciences (ICFIRHS) 2019 (01-May-2019)        |

Journal :   Research Journal of Pharmacy and Technology

Volume No. :   12

Issue No. :  9

Year :  2019

Pages :   4205-4208

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Antibacterial activity for Bacteriocin produced by Lactic acid bacteria against Clinical Bacterial Isolates



Address:   Samer M. Al-Hulu
Environmental Sciences College , Al-Qasim Green University,
*Corresponding Author
DOI No: 10.5958/0974-360X.2019.00723.6

ABSTRACT:
Eight Lactic Acid Bacteria isolates were detected after culturing for 24 dairy samples on MRS medium, and have white-cream color. Isolates were gram positive, rod-cooci shape after examined by microscope. All lactic acid bacterial isolates tested for production of bacteriocin, results showed that L4 isolate having most activity against S. aureus with inhibition zone (14 mm). L4 was identified when cultured on MRS media, it have colonies with white to creamy color and the microscopically test show rod shape bacteria, gram positive, and its negative for motility test. This features belong to Lactobacillus spp. The biochemical test showed the isolate was negative for catalase, indole, oxidase, coagulase. Antibacterial activity of L4 was tested by well diffusion method against S.aureus, E.coli, and P. auroginosa. The result showed that L4 having greatest antibacterial activity with (14mm) inhibition zone. against S.aureus, 12 mm inhibition zone against E. coli, and 10 mm inhibition zone against P. auroginosa.
KEYWORDS:
Lactic Acid Bacteria, bacteriocin production, inhibition zone.
Cite:
Samer M. Al-Hulu. Antibacterial activity for Bacteriocin produced by Lactic acid bacteria against Clinical Bacterial Isolates. Research J. Pharm. and Tech. 2019; 12(9): 4205-4208.
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