2nd International Conference on Fostering Interdisciplinary Research In Health Sciences (ICFIRHS) 2019 (01-May-2019)        |

Journal :   Research Journal of Pharmacy and Technology

Volume No. :   12

Issue No. :  6

Year :  2019

Pages :   2657-2663

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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New Stability Indicating RP-Ultra Fast Liquid Chromatographic Method for the Determination of Perampanel–An Antiepileptic Drug



Address:   Mukthinuthalapati Mathrusri Annapurna*, Rajani Kaibada, Mandula Srinivas
Department of Pharmaceutical Analysis and Quality Assurance GITAM Institute of Pharmacy,
GITAM (Deemed to be University), Visakhapatnam, Andhra pradesh-530045
*Corresponding Author
DOI No: 10.5958/0974-360X.2019.00444.X

ABSTRACT:
A new RP-UFLC method has been developed and validated for the determination of Perampanel in its capsules using Shimadzu Model CBM-20A/20 Alite UFLC system (Shimadzu Co., Kyoto, Japan) equipped with SPD M20A prominence photodiode array detector on C8 (2) 100A (Luna) column (250 mm×4.60 mm i.d. 5µm particle size)maintained at room temperature. Mobile phase consisting of a mixture of water, acetonitrile and 0.1% acetic acid (50:50:0.1 v/v) was chosen with a flow rate of 1 mL/min (UV detection at 215nm) on isocratic mode. Perampanel obeys Beer-Lambert’s law over the concentration range 0.05–120 µg/mL with linear regression equation y=73084x+13800 (r2=0.9999). Forced degradation studies were performed and the method was validated as per ICH guidelines.
KEYWORDS:
Perampanel, RP-UFLC, Stability indicating, Validation, ICH guidelines.
Cite:
Mukthinuthalapati Mathrusri Annapurna, Rajani Kaibada, Mandula Srinivas. New Stability Indicating RP-Ultra Fast Liquid Chromatographic Method for the Determination of Perampanel–An Antiepileptic Drug. Research J. Pharm. and Tech. 2019; 12(6): 2657-2663.
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