2nd International Conference on Fostering Interdisciplinary Research In Health Sciences (ICFIRHS) 2019 (01-May-2019)        |

Journal :   Research Journal of Pharmacy and Technology

Volume No. :   12

Issue No. :  6

Year :  2019

Pages :   2902-2906

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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The Inhibition of Ethanol Extract of Phaleria macrocarpa Stem Bark on COX-2 Expression of HCT116 Colorectal Cancer Cell Line



Address:   Ratu Shafira Hanifah1, Nadia Aida Novitarani1, Fadhilah Harmen1, Aryo Tedjo2,3*,Norma Nur Azizah2,3, Rista Putrianingsih2,3, Wilzar Fachri2,3, Kusmardi Kusmardi3,4
1Medical Student, Faculty of Medicine, Universitas Indonesia, Salemba, Jakarta, Indonesia
2Department of Medical Chemistry, Faculty of Medicine, Universitas Indonesia, Salemba, Jakarta, Indonesia
3Researcher of Cluster Drug Development, Indonesian Medical Education and Research Institute,
Faculty of Medicine, Universitas Indonesia, Salemba, Jakarta, Indonesia
4Department of Pathology Anatomy, Faculty of Medicine, Universitas Indonesia, Salemba, Jakarta, Indonesia
*Corresponding Author
DOI No: 10.5958/0974-360X.2019.00489.X

ABSTRACT:
Objective of this research to determine the inhibition of ethanol extract of Phaleria macrocarpa Boerl. stem bark on COX-2 expression in colorectal cancer cell line HCT116. Ethanol extract of Phaleria macrocarpa stem bark was prepared for maceration in ethanol. Phaleria macrocarpa stem bark in-vitro anticancer activity was tested with MTT assay on HCT116 colorectal cancer cell line. This study also assessed the effect of ethanol extract of Phaleria macrocarpa stem bark on the inhibition of COX-2 expression in the HCT116 colorectal cancer cell line by counting the H-score from immunocytochemistry staining with help from ImageJ and TMARKER application. Ethanol extract of Phaleria macrocarpa stem bark shows anticancer activity by inhibiting its growth with IC50 of 1,327 µg/ml. One of the pathways is through inhibition of COX-2 expression, shown by the H-score of 173,33 after administration of Phaleria macrocarpa stem bark at a dose of 200 ppm. This study shows that Phaleria macrocarpa stem bark is a colorectal cancer growth inhibitor, one of which is through inhibition of COX-2.
KEYWORDS:
COX-2, colorectal cancer, IC50, immunocytochemistry, MTT assay, Phaleria macrocarpa
Cite:
Ratu Shafira Hanifah, Nadia Aida Novitarani, Fadhilah Harmen, Aryo Tedjo, Norma Nur Azizah, Rista Putrianingsih, Wilzar Fachri, Kusmardi Kusmardi. The Inhibition of Ethanol Extract of Phaleria macrocarpa Stem Bark on COX-2 Expression of HCT116 Colorectal Cancer Cell Line. Research J. Pharm. and Tech. 2019; 12(6):2902-2906.
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