2nd International Conference on Fostering Interdisciplinary Research In Health Sciences (ICFIRHS) 2019 (01-May-2019)        |

Journal :   Research Journal of Pharmacy and Technology

Volume No. :   12

Issue No. :  5

Year :  2019

Pages :   2317-2322

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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A Development of An Analytical Spectrophotometeric Method for determination of Taurine in Energy Drinks



Address:   Riham Kais Saoud1*, Fida Am Ali2, Zaid Al Assaf3
1Master Student, Department of Analytical and Food Chemistry, Faculty of Pharmacy, Damascus University, Damascus, Syria
2Professor-Assistant, Department of Analytical and Food Chemistry, Faculty of Pharmacy, Damascus University, Damascus, Syria
3Professor, Department of Analytical and Food Chemistry, Faculty of Pharmacy, Damascus University, Damascus, Syria
*Corresponding Author
DOI No: 10.5958/0974-360X.2019.00386.X

ABSTRACT:
A novel, simple, rapid and sensitive spectrophotometric method has been developed for assay of taurine in energy drinks which is an important amino acid in body human and it is added to energy drinks to improve the mental and muscle performance. The proposed method allows selective determination of taurine in energy drinks, which are a mixture of many components such as caffeine, ginseng, vitamins, guarana, carbohydrates, colorants, preservative agents, etc. Ninhydrin was used as derivatizing agent to form an irreversible complex with taurine. The reaction of taurine with ninhydrin was completed within 20 minutes at 100°C and the colored complex has been measured using spectrophotomer at ?max=568 nm. The optimal conditions of this reaction have been studied. Extraction method has been developed using cation exchange resin to extract taurine and eliminate the interferences of other components in energy drinks. The validation of developed method was performed according to ICH [Q2(R1)] guidelines. A precision was estimated statistically using standard deviation (SD=0.25) and relative standard deviation (RSD%=0.64). The calibration curve was linear with a correlation coefficient (R2 = 0.997). The recoveries were between [98.45-99.33] %. Then fifty samples of consumed energy drinks in Syria have been analyzed using the developed method to determine their content of taurine.
KEYWORDS:
Spectrophotometer, Taurine, Ninhydrin, Validation, Energy drinks.
Cite:
Riham Kais Saoud, Fida Am Ali, Zaid Al Assaf. A Development of An Analytical Spectrophotometeric Method for determination of Taurine in Energy Drinks. Research J. Pharm. and Tech. 2019; 12(5):2317-2322.
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