2nd International Conference on Fostering Interdisciplinary Research In Health Sciences (ICFIRHS) 2019 (01-May-2019)        |

Journal :   Research Journal of Pharmacy and Technology

Volume No. :   12

Issue No. :  5

Year :  2019

Pages :   2182-2184

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Diagnosis of Human Papillomavirus in Colorectal Cancer and Inhibition by Gamma Radiation



Address:   Dr. Fadyia Mahdi Muslim Alameedy
Assistant Professor, Department of Laboratory Investigation, Faculty of Science, Kufa University, Najaf, Iraq
*Corresponding Author
DOI No: 10.5958/0974-360X.2019.00363.9

ABSTRACT:
Human Papillomavirus was is olated from different age groups from 40 upto 71 years for both gender. The samples were diagnosed by RT-PCR. Positive cases of human Papillomavirus were forty represent (63.49%) and twenty two represent (36.50%) negative cases. Isolation process of Human Papilloma virus was successfully performed on chicken embryo fibroblast cell culture for 4th passage for suspected samples obtained from (colon organ) as well as isolate from chick embryo [specific pathogen free (SPF). It was found that at the tissue culture infective dosage (TCID50 /0.1 ml) was (107.8) of virus. The gamma irradiation that dosage used for inactivating of the purified virus which was extracted from allantoic fluid and tissue culture was (6.594) mSv/h. This revision intended to gauge the prevalence of HPV cannon and viral incorporation in colorectal tissues in imperative to estimate its presumed role in colorectal cancer and inhibition viral by gamma radiation.
KEYWORDS:
HPV, RT-PCR, TCID50, SPF.
Cite:
Fadyia Mahdi Muslim Alameedy. Diagnosis of Human Papillomavirus in Colorectal Cancer and Inhibition by Gamma Radiation. Research J. Pharm. and Tech. 2019; 12(5):2182-2184.
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