2nd International Conference on Fostering Interdisciplinary Research In Health Sciences (ICFIRHS) 2019 (01-May-2019)        |

Journal :   Research Journal of Pharmacy and Technology

Volume No. :   12

Issue No. :  5

Year :  2019

Pages :   2137-2140

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Development and Validation of An Analytical Liquid Chromatography-Tandem Mass Spectroscopy Method for the Estimation Febuxostat in Pharmaceutical Formulation



Address:   Lingamallu Venkata Sai Krishna1, Narenderan. S. T1, Ramshankar Nayak1, Meyyanathan. S. N*1, Babu. B1, Kalaivani. M2
1Department of Pharmaceutical Analysis, JSS College of Pharmacy (JSS Academy of Higher Education and Research, Mysuru) Udhagamandalam, India.
2Indian Pharmacopoeia Commission, New Delhi.
*Corresponding Author
DOI No: 10.5958/0974-360X.2019.00354.8

ABSTRACT:
A rapid LC-MS/MS method has been developed and validated for the quantitative determination of Febuxostat from the commercially available formulations. The separation was achieved using Zorbax SB C18 column (4.6 × 50 mm, 5 µm) as a stationary phase and the mobile phase consists of (10 mM) Ammonium formate (pH4.0): methanol (10:90 v/v) with a flow of 0.6 mL/min. Detection was carried out by triple quadrupole mass spectrometry with electrospray ionization in positive mode with proton adducts at m/z 316.95?260.95 to monitor Febuxostat within a run time of 2 min. The linearity of the method was found over a concentration range of 4 to 55 ng/mL with a regression analysis of 0.997. The percentage recovery of the present method was found to be 97.16 to 99.46%. The LC-MS/MS method was validated as per ICH guidelines. The developed method can be successfully applied for the estimation of Febuxostat in the commercial formulation and in bulk drug.
KEYWORDS:
Febuxostat, Formulation, LC-MS/MS, Validation, Electrospray ionization.
Cite:
Meyyanathan. S. N, Babu. B, Kalaivani. M. Development and Validation of An Analytical Liquid Chromatography-Tandem Mass Spectroscopy Method for the Estimation Febuxostat in Pharmaceutical Formulation. Research J. Pharm. and Tech. 2019; 12(5):2137-2140.
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