Emerging Trends in Multidisciplinary Research (ICETMR-2020) 15-16 May 2020 at Dhonburi Rajabhat University, Bangkok, Thailand (07-Feb-2020)        |

Journal :   Research Journal of Pharmacy and Technology

Volume No. :   12

Issue No. :  12

Year :  2019

Pages :   5855-5859

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Detection and Characterization of Cry1ac in BT Cotton Hybrids of MECH 162 and RCH2



Address:   Maheswara Reddy Mallu1*, Nandini Dronavalli1, Sreeja Nannapaneni1, Aishwarya Kamarapu1, Sandeep Vemula2
1Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Andhra Pradesh, India
2Shantha Biotechnics, Hyderabad, Telangana, India
*Corresponding Author
DOI No: 10.5958/0974-360X.2019.01015.1

ABSTRACT:
Background: With the increase market of the transgenic crops, the demand for testing Bt cotton seeds has increased dramatically. ELISA abased protein detection and PCR based gene identification are still major approaches for detecting GMOs on large scale. Objective: In our present study, PCR and ELISA based techniques was adopted to identify the specific Cry1Ac gene and proteins in MECH 162 and RCH2 transgenic cotton plants. Methods: MECH 162 of 21.9ug/g and RCH2 of 17.37ug/g of seed protein was extracted. PCR analysis confirmed that gene specific CRY1Ac F/R primer as amplicon size of 550 bp for Bt gene, 600bp for nptII gene and 200bp for 35s promoter gene. Conclusion: The ELISA method was used for the validation of the developed assay, also ELISA needed simple equipment and took less time.
KEYWORDS:
MECH 162, RCH2, GMO, Cry1Ac, NptII, 35s Promoter.
Cite:
Maheswara Reddy Mallu, Nandini Dronavalli, Sreeja Nannapaneni, Aishwarya Kamarapu, Sandeep Vemula. Detection and Characterization of Cry1ac in BT Cotton Hybrids of MECH 162 and RCH2. Research J. Pharm. and Tech. 2019; 12(12): 5855-5859.
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