2nd International Conference on Fostering Interdisciplinary Research In Health Sciences (ICFIRHS) 2019 (01-May-2019)        |

Journal :   Research Journal of Pharmacy and Technology

Volume No. :   12

Issue No. :  12

Year :  2019

Pages :   5811-5814

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Simultaneous Equation Method for Estimation of Rutin Trihydrate and Berberine Chloride in Synthetic Mixture and Polyherbal Formulation



Address:   Dr Palak Chaudhary*, Dr. Harsha U. Patel
Shri Satsangi Saketdham “Ram Ashram”, Group of Institutions, Faculty of Pharmacy,
Vadasma, Mehsana, Gujarat, India.
*Corresponding Author
DOI No: 10.5958/0974-360X.2019.01006.0

ABSTRACT:
The present study was focused on establishing and validating a simple, accurate, reliable and economical UV spectrophotometric method for the simultaneous determination of Rutin trihydrate (RUT) and Berberine Chloride (BER) in synthetic mixture and in antidiabetic polyherbal formulation. Rutin and Berberine from herbal plants are used as antidiabetic drug to prevent and manage Diabetes. The developed method gives best results in terms of linearity, accuracy, precision, limit of detection (LOD) and limit of quantification (LOQ). The method was validated as per ICH guidelines. The linearity range for Rutin trihydrate and Berberine Chloride were found to be 10 to 50 µg/ ml and 10 to 50 µg/ml respectively. The values of LOD were 0.062 µg/ ml and 0.076 µg/ml and the values of LOQ were found to be 0.187 µg /ml and 0.229 µg/ ml for Rutin trihydrate and Berberine chloride respectively.
KEYWORDS:
Rutin trihydrate (RUT), Berberine chloride (BER), Simultaneous equation method, Validation, Polyherbal formulation.
Cite:
Palak Chaudhary, Harsha U. Patel. Simultaneous Equation Method for Estimation of Rutin Trihydrate and Berberine Chloride in Synthetic Mixture and Polyherbal Formulation. Research J. Pharm. and Tech. 2019; 12(12): 5811-5814.
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