2nd International Conference on Fostering Interdisciplinary Research In Health Sciences (ICFIRHS) 2019 (01-May-2019)        |

Journal :   Research Journal of Pharmacy and Technology

Volume No. :   12

Issue No. :  10

Year :  2019

Pages :   4790-4794

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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New first derivative spectrophotometric methods for the estimation of Bumetanide in tablet dosage forms



Address:   Mukthinuthalapati Mathrusri Annapurna*, Avuthu Sai Sheela, Rangisetty Spandana Yasaswini
Department of Pharmaceutical Analysis & Quality Assurance, Gandhi Institute of Technology and Management, GITAM Institute of Pharmacy, GITAM (Deemed to be University), Visakhapatnam, Andhra Pradesh-530045, India.
*Corresponding Author
DOI No: 10.5958/0974-360X.2019.00827.8

ABSTRACT:
First derivative spectrophotometric methods have been developed for the assay of Bumetanide using Shimadzu UV-1800 Model double beam UV-VIS spectrophotometer in reagents such as hydrochloric acid, phosphate buffer pH 3.6, phosphate buffer pH 2.0 and distilled water. Bumetanide has shown linearity 10-70 µg/ml in all the methods and the linear regression equations are found to be y = 0.0015x - 0.0013 (R² = 0.9993), y= 0.0007x - 0.0004 (R² = 0.9991), y = 0.0014x - 0.0015 (R² = 0.9993) and y = 0.0014x - 0.0015 (R² = 0.9995) in hydrochloric acid, phosphate buffer pH 3.6, phosphate buffer pH 2.0, and distilled water respectively. The methods are validated as per ICH guidelines and found to be precise, simple and accurate.
KEYWORDS:
First order derivative spectroscopy (D1), Bumetanide, Hydrochloric acid, Phosphate buffer, Validation, ICH guidelines.
Cite:
Mukthinuthalapati Mathrusri Annapurna, Avuthu Sai Sheela, Rangisetty Spandana Yasaswini. New first derivative spectrophotometric methods for the estimation of Bumetanide in tablet dosage forms. Research J. Pharm. and Tech 2019; 12(10):4790-4794.
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