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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   11

Issue No. :  9

Year :  2018

Pages :   4157-4160

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Visible Spectrophotometric estimation of Simeprevir in Pharmaceutical Formulations

Address:   Nakka Raju*1, P. Raveendra Reddy2, P. Janaki Pathi3, N. Appala Raju4
1,2Department of Chemistry, Sri Krishnadevaraya University, Anantapur-515 003
3Analytical Department, Vishnu Chemicals Limited, Jeedimetla, Hyderabad.
4Department of Pharmaceutical Chemistry, Sultan-Ul-Uloom College of Pharmacy Mount Pleasant, Hyderabad-500 034.
*Corresponding Author
DOI No: 10.5958/0974-360X.2018.00763.1

Two simple, accurate, rapid and sensitive methods (A and B) have been developed for the estimation of Simeprevir in its pharmaceutical dosage form. The Method A is based on the formation of bluish-green colored chromogen, due to reaction of Simeprevir with Folin–Ciocalteu reagent (FCR) reagent in the presence of alkaline medium, which exhibits ? max at 715 nm. Method B is based on the reaction of Simeprevir with 3-methyl-2-benzothiazolinone hydrazone (MBTH) in the presence of Ferric chloride to form a green colored chromogen, which shows maximum absorbance at 645 nm. The absorbance-concentration plot is linear over the range of 50-500 µg/mL for Method A, 10-100 µg/mL for Method B. Results of analysis for all the methods were validated statistically and by recovery studies. Literature survey reveals only HPLC methods deals with biological fluids and in fixed pharmaceutical dosage forms. The proposed methods are economical and sensitive for the estimation of Simeprevir in bulk drug and in its formulations.
UV-Visible Spectrophotometry, Simeprevir, Ferric chloride, Folin–Ciocalteu reagent (FCR), 3-methyl 2-benzothiazolinone hydrazone, (MBTH), Sodiumbicarbonate.
Nakka Raju, P. Raveendra Reddy, P. Janaki Pathi, N. Appala Raju. Visible Spectrophotometric estimation of Simeprevir in Pharmaceutical Formulations. Research J. Pharm. and Tech 2018; 11(9): 4157-4160.
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