Journal :   Research Journal of Pharmacy and Technology

Volume No. :   11

Issue No. :  9

Year :  2018

Pages :   3874-3880

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Effects of Glycosaminoglycans Extrat from sea Cucumber Stichopus vastus on Inflammatory Mediators and Tissue Healing on Rodents with full Thickness Burn Injury



Address:   Mohd Faeiz Pauzi1, Siti Norazlina Juhari1, Mohd Nikman Ahmad2, Farid Che Ghazali2
1Medical Faculty, Universiti Sultan Zainal Abidin, Medical Campus, Jalan Sultan Mahmud, 20400 Kuala Terengganu, Malaysia.
2 Medical Campus, Universiti Sains, Malaysia,16150 Kubang Kerian, Malaysia.
*Corresponding Author
DOI No: 10.5958/0974-360X.2018.00710.2

ABSTRACT:
This study was design to investigate the effect of total sulfated GAG from integument body wall of sea cucumber Stichopus vastus on inflammatory mediators and tissue healing on rodents with full thickness burn injury.Twelve male Sprague-dawley rats weighting 350-400 grams were distributed randomly into two groups. Six rats in group A received 5 ml/kg/day intraperitoneal total sulfated GAG from S.vastusfor 5 days, while control group received phosphate buffered saline (PBS). Level ofIL-10 and IL-12 were measured at day 1, 6 and 12. The level of IL-10 revealed significantly (p<0.05) higher andthe level of IL-12 was reduced significantly (p<0.05) in sulfated GAG treated group. Data suggested that sulfated GAG from S. vastus integument body wall increase anti-inflammatory cytokine and reduce pro-inflammatory cytokine. Microscopic evaluation showed reduced neutrophils infiltration with enhanced proliferation of fibroblasts and angiogenesis (p<0.05) in the sulfated GAG treated group. Sulfated GAG from S. vastus integument body wall has impact on the outcome of wound healing process by increasing the level of anti-inflammatory cytokine, IL-10 and reduced level of pro-inflammatory cytokine, IL-12 and positive effect on fibroblast proliferation and angiogenesis process.
KEYWORDS:
Glycosaminoglycans, GAG, sea cucumber, inflammatory mediators, tissue healing
Cite:
Mohd Faeiz Pauzi, Siti Norazlina Juhari, Mohd Nikman Ahmad, Farid Che Ghazali. Effects of Glycosaminoglycans Extrat from sea Cucumber Stichopus vastus on Inflammatory Mediators and Tissue Healing on Rodents with full Thickness Burn Injury. Research J. Pharm. and Tech 2018; 11(9): 3874-3880.
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