2nd International Conference on Fostering Interdisciplinary Research In Health Sciences (ICFIRHS) 2019 (01-May-2019)        |

Journal :   Research Journal of Pharmacy and Technology

Volume No. :   11

Issue No. :  9

Year :  2018

Pages :   3826-3832

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Phenolic Content, HPLC Analysis and Antioxidant Activity Extract from Tamarix gallica and Tamarix articulata Growing in Southeast of Algeria



Address:   Salemi Said1,3, Gherraf Noureddine2, Laouini Salah Eddine3*, Guerram Abdelmadjid3, Berrani Djamel3, Ali Tliba4
1Department of Chemistry, Faculty of Sciences Matter and Mathematics, University of Kasdi Merbah Ouargla, Algeria
2Laboratory of Biomolecules and Plant Breeding, Life Science and Nature Department, University of Larbi Ben Mhidi, Oum El Bouaghi, Algeria
3Department of Process Engineering and Petrochemistry , Faculty of Technology, Hamma Lakhdar University, El Oued, Algeria
4Laboratory of Valorization and Technology of Saharian Resources, Hamma Lakhdar University, El-Oued, Algeria
*Corresponding Author
DOI No: 10.5958/0974-360X.2018.00701.1

ABSTRACT:
This work reports the phytochemical profile phenolic content and flavonoid, in vitro antioxidant against DPPH, ß-carotene radical and total antioxidant activity of methanolic leaves extract from Tamarix gallica and Tamarix articulata. Formore, HPLC analysis for identification of individual polyphenols was identification in different phenolic groups and the quantification are also described. The quantification of total phenolic and flavonoids content realized by spectrophotometric methods. The antiradical capacities of extracts were evaluated against DPPH free radical and phosphomolybdenum method. Total phenolic contents and flavonoid from an extract of Tamarix gallica were founded high against Tamarix articulata, 334.19±8.47, 395.62±6.23 mg GAE/g DW for phenolic content and 159.73±6.28, 117.47±4.04 mg CE/g DW respectively. The HPLC analysis showed that at least 6 considerable phenolic compounds of leaves extract exist for the two extracts, the major ones being vanillic acid, naringin, and caffeic acid. Similar results for antioxidant activity leaves extract from Tamarix gallica exhibited strong inhibition of free radical against DPPH against Tamarix articulata IC50=5.70±0.18, 7.69±0.11 µg/ml and total antioxidant activity 341.69±9.28, 287.64±8.72 mg GAE/g DW respectively. The obtained results suggest that Tamarix gallica and Tamarix articulata could be a promising source for new drugs because of its antioxidant properties and phenolic compounds.
KEYWORDS:
Tamarix gallica, Tamarix articulata, Phenolic content, Flavonoid, HPLC, DPPH, antioxidant.
Cite:
Salemi Said, Gherraf Noureddine, Laouini Salah Eddine, Guerram Abdelmadjid, Berrani Djamel, Ali Tliba. Phenolic Content, HPLC Analysis and Antioxidant Activity Extract from Tamarix gallica and Tamarix articulata Growing in Southeast of Algeria. Research J. Pharm. and Tech 2018; 11(9): 3826-3832.
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