2nd International Conference on Fostering Interdisciplinary Research In Health Sciences (ICFIRHS) 2019 (01-May-2019)        |

Journal :   Research Journal of Pharmacy and Technology

Volume No. :   11

Issue No. :  7

Year :  2018

Pages :   2965-2970

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Diagnosis and Distinguishing of Entamoeba spp. by PCR Technique



Address:   Najim Abd Al-Wahid Al-Hassany, Ahmed Hieran Ramadhan Al-Jabery
Department of Biology, College of Science, University of Al-Qadisiyah, Iraq
*Corresponding Author
DOI No: 10.5958/0974-360X.2018.00547.4

ABSTRACT:
This study conducted between October 2016 to September 2017, with the aims of distinguishing between amoeba species by molecular methods and determination of the prevalence of pathogenic and non-pathogenic species, In Imam Hussein Teaching Hospital, in the province of ThiQar southern Iraq. 96 stool samples were positive in microscopic examination. After the PCR test, positive samples were found in 86 samples and 10 negative samples E.histolytica were the most common 82 (85.4%) Followed by E.dispar14 (14.5%) These two parasites mixed infection E.histolytica+E.dispar 10 (10.4%) cases, and E.histolytica+ E. hartmanni 8 (8.3%) cases.
KEYWORDS:
Diagnosis, DNA Sequance, Entamoeba spp., PCR technique, phylogenetic tree.
Cite:
Najim Abd Al-Wahid Al-Hassany, Ahmed Hieran Ramadhan Al-Jabery.Diagnosis and Distinguishing of Entamoeba spp. by PCR Technique. Research J. Pharm. and Tech 2018; 11(7): 2965-2970.
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