2nd International Conference on Fostering Interdisciplinary Research In Health Sciences (ICFIRHS) 2019 (01-May-2019)        |

Journal :   Research Journal of Pharmacy and Technology

Volume No. :   11

Issue No. :  2

Year :  2018

Pages :   537-542

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Isolation and Characterization of Exopolysaccharides FromYeast Isolates



Address:   Priyanka Prakash Kharat, Sarita RamsaranYadav, Mangala Lakshmi Ragavan, Nilanjana Das*
Bioremediation Laboratory, School of Bio Sciences and Technology, VIT University, Vellore-632014, Tamil Nadu, India.
*Corresponding Author
DOI No: 10.5958/0974-360X.2018.00100.2

ABSTRACT:
Exopolysaccharides (EPS) are high molecular weight polymers which are long chain composed of sugar residues and secreted by microbes into the surrounding environment. Sugar compositions of exopolysaccharides are dependent to culture medium composition and different physical conditions.Bacteria and fungi are most common EPS producers . In this study, we aimed to isolate the yeasts from different sources for maximum EPS production using optimized conditions. Yeast isolates PS1 and PS2wereanalyzed for maximum EPS production on modified EPS media and optimized physical conditions such as carbon source, temperature and incubation duration. The maximum EPS (0.2 g/L) production was noted in PS1 and PS2.Characterization of purified EPS was done by HPLC, FT-IR and viscometer. PS1 (0.33mg/ml) and PS2 (0.28 mg/ml) showed increased carbohydrate content andsimilarly protein content also quantified for PS1(0.29µg/ml) and PS2 (0.180µg/ml) which indicates glycoprotein quantity on EPS. PS1showed 3% viscosity than PS2. We demonstrate the biological activities of purified EPS viz. Surfactant activity, flocculant activity and antioxidant activities.All these activities are observed in PS1; incaseof PS2 it is not observed. In conclusion, yeast (PS1) exopolysaccharideshas a potential role in the textile, pharmaceutical and cosmetics industries.
KEYWORDS:
Antioxidantactivity, biosurfactant, HPLC, viscosity, yeast exopolysaccharides.
Cite:
Priyanka Prakash Kharat, Sarita Ramsaran Yadav, Mangala Lakshmi Ragavan, Nilanjana Das. Isolation and Characterization of Exopolysaccharides FromYeast Isolates. Research J. Pharm. and Tech 2018; 11(2):537-542.
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