Journal :   Research Journal of Pharmacy and Technology

Volume No. :   11

Issue No. :  12

Year :  2018

Pages :   5273-5281

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Application of Response Surface Methodology in Process Parameter Optimization of Media for Production of Amylase



Address:   R. Subbaiya1*, S. Priyanka2, D. Suresh3, M. Masilamani Selvam4, R. Balachandar5 S. Chozhavendhan1 1Department of Biotechnology, Aarupadai Veedu Institute of Technology, Vinayaga Mission’s Research Foundation, Paiyanoor, Chennai – 603104
2Department of Biotechnology, K. S. Rangasamy College of Technology, Tiruchengode – 637215
3Department of Microbiology, School of Life Sciences, Vels Institute of Science, Technology and Advanced Studies (VISTAS), Pallavaram, Chennai
4Department of Biotechnology, Sathyabama Institute of Science and Technology, Jeppiaar Nagar, Chennai–600119
5Department of Biotechnology, Arulmigu Meenakshi Amman College of Engineering, Vadamavandal, Kanchipuram – 604410
*Corresponding Author
DOI No: 10.5958/0974-360X.2018.00961.7

ABSTRACT:
Agricultural wastes are being discarded and those wastes get accumulated in the environment. On the other hand, amylase production has become significant for its use in various industries like food, fermentation, brewing, detergent and paper industries. For this elevating demand, amylase production has to be increased. By using the agricultural waste as substrate source amylase enzyme can be produced in higher quantities. Using Bacillus subtilis as microbial source, agricultural waste can be utilised for the production of amylase in submerged fermentation as well as in solid state fermentation. Usage of synthetic media is very expensive and hence normal nutrient broth can be used. By using OVAT procedure, parameters such as temperature, pH, and substrate concentration can be varied and the optimum level at which enzyme production is higher can be determined. Response surface methodology is used for efficient production of enzyme. The enzyme produced in this process could be useful in reducing the demand of amylase in commercial use.
KEYWORDS:
Amylase, OVAT, Submerged Fermentation, Response Surface Methodology.
Cite:
R. Subbaiya, S. Priyanka, D. Suresh, M. Masilamani Selvam, R. Balachandar S. Chozhavendhan. Application of Response Surface Methodology in Process Parameter Optimization of Media for Production of Amylase. Research J. Pharm. and Tech 2018; 11(12): 5273-5281.
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