Journal :   Research Journal of Pharmacy and Technology

Volume No. :   11

Issue No. :  12

Year :  2018

Pages :   5221-5228

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Determination of Antioxidant Capacity, Flavonoids and Total Phenolic Content of Extracts from Atractylis flava Desf



Address:   Mohamed Akram Melakhessou*, Naima Benkiki, Salah Eddine Marref
Laboratoire de Biotechnologie des Molécules Bioactives et Physiopathologie Cellulaire, Faculté des Sciences de la Nature et de la vie, Département de Biologie des Organismes, Université de Batna 2, Batna 05000, Algérie.
*Corresponding Author
DOI No: 10.5958/0974-360X.2018.00952.6

ABSTRACT:
objective: The aim of the present study was to evaluate the ability of different extracts (PE, DCM, EtAOc and n-BuOH) obtained from Atractylis flava Desf to prevent oxidation using several methods in vitro. Materials and Methods: The capacity of samples for scavenging free radicals (ABTS+, DPPH), preventing lipid peroxidation (ß-carotene), chelating metal ions, CUPRAC, reducing power. The quantification of total phenols was performed using Folin-Ciocalteu reagent and the total flavonoid content was performed by the trichloroaluminum method. Results: The highest concentrations of total polyphenols and flavonoids were found in the n-BuOH extract (122,74 ± 0,78 µg GAE/ml and 66,75±6,12 µg QE/ml, respectively). EtAOc and n-BuOH extracts were the most active for scavenging DPPH (IC50 = 158,41±3,24 and IC50 = 427,53±4,69 µg/mL, respectively), and EtAOc extract was also the best for scavenging ABTS+ radicals (IC50 = 15,57±2,93 µg/mL). EtAOc extract possessed the best capacity for preventing lipid peroxidation (IC50 = 16,52±1,87 µg/mL) and CUPRAC assay (A0.50 = 28,58±0,65 µg/mL). The results of ferric reducing power ability of all the tested samples were affected in a dose-dependent manner. Conclusions: These results suggest that Atractylis flava Desf have strong antioxidant potential. Further study is necessary for isolation and characterization of t of the active antioxidant agents, which can be used to treat various oxidative stress-related diseases.
KEYWORDS:
Antioxidant, Atractylis flava Desf, polyphenols, flavonoids.
Cite:
Mohamed Akram Melakhessou, Naima Benkiki, Salah Eddine Marref. Determination of Antioxidant Capacity, Flavonoids and Total Phenolic Content of Extracts from Atractylis flava Desf. Research J. Pharm. and Tech 2018; 11(12): 5221-5226.
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