Journal :   Research Journal of Pharmacy and Technology

Volume No. :   11

Issue No. :  10

Year :  2018

Pages :   4365-4369

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Screening of Marine Actinomycetes for Fibrinolytic Enzymes



Address:   K. Viswanathan*, L. Jeyanthi Rebecca
Department of Industrial Biotechnology, Bharath Institute of Higher Education and Research, Chennai, Tamilnadu, India
*Corresponding Author
DOI No: 10.5958/0974-360X.2018.00799.0

ABSTRACT:
Objective: This study aims to isolate the potential fibrinolytic enzyme from actinomycetes strains. Methods: Screening of actinomycetes from marine soil samples by using starch casein agar plate method, Protease activity by skim milk agar plate method and fibrinolytic enzyme activity by Fibrin plate method. Results: We have isolated 114 actinomycetes strains by starch casein agar plate method and the morphology was identified by coverslip method. Out of the 114 actinomycetes strains, 40 actinomycetes strains showed protease activity by skim milk agar plate method. Protease producing actinomycetes strains were tested for fibrinolytic activity by Fibrin plate method which was indicated by growth and clear zone of fibrinolysis. Out of 40 isolates, the fibrinolytic activity was highest in 12 actinomycetes strains. Conclusion: Based on the maximum fibrinolytic activity, BN22 was found to be a potential strain for further investigation.
KEYWORDS:
Actinomycetes, Protease activity, Fibrinolytic activity.
Cite:
K. Viswanathan, L. Jeyanthi Rebecca. Screening of Marine Actinomycetes for Fibrinolytic Enzymes. Research J. Pharm. and Tech 2018; 11(10): 4365-4369.
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