Journal :   Research Journal of Pharmacy and Technology

Volume No. :   11

Issue No. :  10

Year :  2018

Pages :   4267-4271

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Sequencing of Mitochondrial COI gene in new Iraqi record Paropeas achatinaceum L. P. Feiffer, 1846, Gastropoda: Subulinidea



Address:   Ihsan A. Hussein, Nibras L. Aldoori, Maysaloon L. Aldoori*
Department of Biology, College of Education for Pure Science- Ibn-Al-Haitham, University of Baghdad, Baghdad, Iraq
*Corresponding Author
DOI No: 10.5958/0974-360X.2018.00782.5

ABSTRACT:
Fifty snails of Paropeas achatinaceum specimens were collected and classified from four areas in Baghdad-Iraq from the period between June and July, 2017. The snails were divided into two groups (each group contain 25 snails). Two environment conditions were used in this study. Natural environment considered as control and experimental environment contains Citrus sinensis (L.) roots as snail’s source food. The comparison result between snail weights in the nature and experimental environment was not significant (0.497, 95% confidence interval [CI] 0.01209–0.02309). Also, the comparison between snail weights in the nature environment and the food mean weight was significant (0.014, 95% confidence interval [CI] 0.00591-0.04109), while the comparison between snail weights in the experimental environment and the food mean weight was significant (0.005, 95% confidence interval [CI] 0.01141-0.04659). Sequencing of COI gene of this specimen was done to find the similarity with the same gene sequences database which published in National Center of Biotechnology Information (NCBI). Polymerase chain reaction (PCR) technique revealed presence one band for part of mt DNA COI gene with 710 bp. The sequencing results revealed a high similarity (98%) with the Paropeas achatinaceum (accession number MF415354.1; specimen collected from Sri Lanka), which published in NCBI. The sequence data was submitted in the NCBI and the accession number of the COI gene sequences was MG747646.1.
KEYWORDS:
Paropeas achatinacium, Gastropoda, Subulinidae, Mollusca, COI gene, PCR
Cite:
Ihsan A. Hussein, Nibras L. Aldoori, Maysaloon L. Aldoori. Sequencing of Mitochondrial COI gene in new Iraqi record Paropeas achatinaceum L. P. Feiffer, 1846, Gastropoda: Subulinidea. Research J. Pharm. and Tech 2018; 11(10): 4267-4271.
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