List of Refereed Journals published by UGC, New Delhi on 28-03-2017-- AJRC (Sr. No.-1025); AJM (Sr. No.-2497); RJET (Sr. No.-7220); RJPP (Sr. No.- 7232): RJPPD (Sr. No.- 7234); RJPT (Sr. No.-7235) (19-Apr-2017)        | List of Refereed Journals published by UGC, New Delhi on 11-01-2017-- Research Journal of Pharmacy and Technology (Sr. No.-275); Asian Journal of Management (Sr. No.-3986); Asian Journal of Research in Chemistry (Sr. No.-4008); Research Journal of Engineering and Technology (Sr. No.-31787); Research (22-Jan-2017)        |

Volume No. :   10

Issue No. :  3

Year :  2017

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Isolation, Identification and In vitro Characterisation of Probiotic Yeast Strains



Address:   Abhirup Dey, Mangala Lakshmi Ragavan, Sanjeeb Kumar Mandal, Nilanjana Das*
Bioremediation Laboratory, School of Bio Sciences and Technology, VIT University, Vellore-632014, Tamil Nadu, India.
*Corresponding Author
DOI No: 10.5958/0974-360X.2017.00136.6

ABSTRACT:
In the present study, ten yeasts were isolated from fruit juices and subjected to in vitro tests for screening probiotic potentiality. Three isolates exhibited higher tolerance for pH and heat stress. Based on preliminary screening, isolates were subjected to molecular identification and identified as Wickerhamomyces anomalusVIT-ASN01, Saccharomyces cerevisiae VIT- ASN03 and Yarrowia lipolytica VIT- ASN04. The screened yeast isolates survived and grew well at human gastrointestinal conditions in pH 2.5 and 0.3% (w/v) oxgall at 37°C. The antagonistic activity of yeast isolates were tested against clinical pathogens, viz.S. typhii, B. subtilis and E. coli. W. anomalusVIT-ASN01 showed maximum tolerance to high bile salt concentration and acidity, could remove cholesterol 51.28% during 48 h and produced 586.55 mg/L of exopolysaccharide. It can be concluded that the screened novel yeast isolates can serve as promising probiotics in various fields of food industry.
KEYWORDS:
Bile tolerance, cholesterol assimilation, probiotics, yeast.
Cite:
Abhirup Dey, Mangala Lakshmi Ragavan, Sanjeeb Kumar Mandal, Nilanjana Das. Isolation, Identification and In vitro Characterisation of Probiotic Yeast Strains. Research J. Pharm. and Tech. 2017; 10(3): 726-732.
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