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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   10

Issue No. :  12

Year :  2017

Pages :   4231-4236

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Detection of Efflux Pumps Genes in Clinical Isolates of Acinetobacter baumannii



Address:   Raad Abdulabass AL-Harmoosh1*, Eman M. Jarallah2, Anmar M. AL-Shamari3, Hussein M. AL-Khafaji4
1University of Kufa, Faculty of Science, Najaf, IRAQ
2University of Babylon, Faculty of Science, Babylon, IRAQ
3Ministry of Health and Environment, Babylon Health and Environment Office, Babylon, IRAQ.
4Ministry of Health and Environment, Babylon Health Office, Babylon, IRAQ.
*Corresponding Author
DOI No: 10.5958/0974-360X.2017.00775.2

ABSTRACT:
One of the most interesting features of Acinetobacter baumannii is the ease by which it can acquire resistance to various antibiotics. Mutations, new gene acquisitions, up-regulating expression of existing genes (should those be genes of drug-destroying enzymes or efflux pumps), or losing them, like in the case of porins, all may contribute to the broad spectrum resistance of these strains.This study was directed to decide the event of efflux pumpsgenes harboring A. baumannii isolates recovered from Babylon hospitals. Isolates were recognized by biochemical tests and more affirmed utilizing API 20E system. Antibioticssusceptibility was measured by utilizing disks diffusion test. Ten (0.76%) A. baumannii isolates were recovered from clinical specimens. One (10%) isolate was observed to be imipenem and meropenem resistant (MIC > 512 µg/ml). There are indicative appearance of efflux pump genes ; (50%) for Ade-A and Ade-B each other , (40%) for Ade-C, (80%) for all of Ade-R and Ade-S , (100%) for all of Ade-I and Ade-J , and (50%) for Ade-Y. All isolates of A. baumannii appear as MDR , while only one appear to be as PDR.
KEYWORDS:
Efflux pump, Ade-ABC, Acinetobacter, Carbapenem, Ade-RS, Ade-IJ).
Cite:
Raad Abdulabass AL-Harmoosh, Eman M. Jarallah, Anmar M. AL-Shamari, Hussein M. AL-Khafaji. Detection of Efflux Pumps Genes in Clinical Isolates of Acinetobacter baumannii . Research J. Pharm. and Tech 2017; 10(12): 4231-4236.
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