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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   10

Issue No. :  11

Year :  2017

Pages :   3990-3996

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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RP-HPLC Method for Simultaneous Estimation of Cilnidipine and Chlorthalidone



Address:   Vijaykumar T. Pawar1, Shubhangi V. Pawar2, Harinath N. More1, Anita S. Kulkarni3, Dinanath T. Gaikwad4
1Department of Pharmaceutical Chemistry, Bharati Vidyapeeth College of Pharmacy, Near Chitranagari, Kolhapur-416013, M. S., India
2Department of Pharmaceutical Chemistry, Shri. Santkrupa College of Pharmacy, Ghogaon
3Department of Pharmaceutical Chemistry, Government College of Pharmacy, Karad
4Department of Pharmaceutics, Bharati Vidyapeeth College of Pharmacy, Near Chitranagari, Kolhapur-416013, M. S., India
*Corresponding Author
DOI No: 10.5958/0974-360X.2017.00724.7

ABSTRACT:
Aim of present study was development of RP-HPLC method for simultaneous estimation of cilnidipine and chlorthalidone. RP-HPLC method for simultaneous estimation of Cilnidipine and Chlorthalidone was carried out by using methanol: water (80:20 v/v) at a flow rate of 1 ml min-1 with UV detection at 231.6 nm. Both drugs showed linearity in the concentration range of 10-70 µg ml-1.for CIL and 10-70 µg ml-1.for CHLT. When marketed pharmaceutical preparations were analyzed, the result obtained by the proposed method was found in good agreement with labeled claims. Recoveries in all these method were in the range of 99.60-100.18%. Results obtained were statistically validated and were found to be reproducible. These methods can be applied for routine analysis of formulations containing these drugs. For analytical methods validation parameters such as linearity, precision, accuracy, specificity, limit of detection, limit of quantization and robustness were determined according to ICH Q2B guidelines.
KEYWORDS:
Cilnidipine, Chlorthalidone; RP-HPLC; ICH Q2B, Validation.
Cite:
Vijaykumar T. Pawar, Shubhangi V. Pawar, Harinath N. More, Anita S. Kulkarni, Dinanath T. Gaikwad. RP-HPLC Method for Simultaneous Estimation of Cilnidipine and Chlorthalidone. Research J. Pharm. and Tech 2017; 10(11): 3990-3996.
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