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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   10

Issue No. :  10

Year :  2017

Pages :   3561-3566

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Industrial Purification Strategies for Monoclonal Antibodies



Address:   Narendra Kumar S1*, Lingayya Hiremath1, Praveen Kumar Gupta1, Ajeet Kumar Srivastava1, Poornima S4
1Assistant Professor, Department of Biotechnology, R V College of Engineering, Bangalore
2PG-Student, M. Tech Biotechnology, R V College of Engineering, Bangalore-560059
*Corresponding Author
DOI No: 10.5958/0974-360X.2017.00645.X

ABSTRACT:
The global monoclonal antibody market in 2016 was around USD 85.4 billion and is expected to reach USD 140 billion by end of 2024 i.e., approximately 1.5 fold increase in market rate within 10 years. The therapeutics purification process monoclonal antibody through chromatography technique is expected to grow by 5% from 2011 to 2017, with a prediction of reaching to USD 9 billion by end of 2017. The global market interest is shifting from monoclonal antibody to fragmented antibody; nearly 60 fragmented antibodies have gone into clinical trials as of 2010. In this review, recovery of monoclonal antibody by centrifugation, depth filtration followed by purification by chromatography techniques i.e., capture step by affinity chromatography, intermediate step by anionic chromatography in flow through mode and polishing step by cationic chromatography is reviewed. The production of antigen binding fragment is carried out by enzymatic method followed by affinity chromatography, multimode chromatography and cationic chromatography. Monoclonal antibodies have been developed by these methods are used for targeting range of diseases. Many of such monoclonal antibodies have been approved and many such is still in development pipeline. This review gives an overview on the structure of antibody, production of monoclonal antibody and the downstream purification of monoclonal antibody. The downstream purification covers the basic techniques followed for most of the mAb purification done commercially. The downstream purification steps discussed here include centrifugation, tangential flow filtration, depth filtration and chromatographic techniques.
KEYWORDS:
Monoclonal Antibody, Downstream purification, Centrifugation, Ion Exchange chromatography.
Cite:
Narendra Kumar S, Lingayya Hiremath, Praveen Kumar Gupta, Ajeet Kumar Srivastava, Poornima S. Industrial Purification Strategies for Monoclonal Antibodies. Research J. Pharm. and Tech 2017; 10(10):3561-3566.
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