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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   9

Issue No. :  3

Year :  2016

Pages :   245-250

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Isolation of α-Amylase Producing Bacteria from Naini lake and Assessment of their Anti-Oxidant and anti-Fungal Properties



Address:   Shamalee Nitin Narappanawar, Shruti Sunil Ranade, Swarnita Kandpal, Veena S , K.V. Bhaskara Rao*
Molecular and Microbiology Laboratory, School of Biosciences and Technology, VIT University, Vellore-632014, India
*Corresponding Author
DOI No: 10.5958/0974-360X.2016.00045.7

ABSTRACT:
Bacterial enzymes play an important role in the industry. The objective of this study was to isolate and screen a-amylase producing bacteria from water samples obtained from various regions of the Naini Lake, Uttarakhand, India. A total of 11 bacterial isolates were obtained on nutrient agar medium, which were then screened for the production of a-amylase. Antioxidant and anti-fungal property of the bacterial isolates which tested positive for a-amylase production was also assessed. Bacterial isolates NLWS 5 and NLWS 7 showed the highest enzyme production with a zone of 14mm and 10mm respectively. These two strains showed anti-oxidant as well as anti-fungal activity which were then selected for identification using Bergey’s Manual of Determinative Microbiology. It was found that the bacterial isolates which showed the highest amount of a-amylase production, anti-oxidant and anti-fungal activity belonged to Corynebacterium sp.
KEYWORDS:
Bacterial isolates, Naini Lake, α-Amylase, anti-oxidant property, anti-fungal property, Corynebacterium sp.
Cite:
Shamalee Nitin Narappanawar, Shruti Sunil Ranade, Swarnita Kandpal, Veena S, K.V. Bhaskara Rao. Isolation of α-Amylase Producing Bacteria from Naini lake and Assessment of their Anti-Oxidant and anti-Fungal Properties. Research J. Pharm. and Tech. 9(3): Mar., 2016; Page 245-250.
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