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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   9

Issue No. :  10

Year :  2016

Pages :   1638-1640

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Detection of the presence of blaTEM-1gene for ESBL production among clinical strains of E. coli.



Address:   R. Monalisa, Dr. Gopinath
Saveetha Dental College and Hospitals No. 162, PH Road, Chennai-600077
*Corresponding Author
DOI No: : 10.5958/0974-360X.2016.00328.0

ABSTRACT:
Majority of ESBLs are derived from the amino acid substitutions of their parent enzymes TEM and SHV ß-lactamases, but there are several other types of acquired ESBLs found in Enterobacteriaceae. Among these ESBLs, TEM, SHV and CTX-M types of are of being major clinical concern and predominated in Gram-negative bacteria. In this study we have taken 20 clinical isolates of E.coli and were subjected to antibiotic sensitivity pattern followed by the detection of blaTEM-1gene by PCR. We have observed total resistance to cephalosporins followed by other group of drugs. 20% of our strains were found to have this gene. This indicates that apart from this gene there several other genes that encodes for ESBLs such as CTX-M, SHVetc may be the reason for showing the resistance. To rule out this more number of isolateshave to be included with a battery of other genes to be screened to get the actual outcome.
KEYWORDS:
E. coli, PCR, blaTEM-1gene
Cite:
R. Monalisa, Gopinath. Detection of the presence of blaTEM-1gene for ESBL production among clinical strains of E. coli. Research J. Pharm. and Tech 2016; 9(10):1638-1640.
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