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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   9

Issue No. :  10

Year :  2016

Pages :   1626-1628

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Detection of CNA Gene for the Presence of Collagen Adhesion in Clinical Strains of Staphylococcus aureus



Address:   S.V. Rupashri1, Dr. P. Gopinath2
1BDS 2nd year, Saveetha Dental College, Chennai.
2Senior Lecturer, Department of Microbiology, Saveetha Dental College, Chennai.
*Corresponding Author
DOI No: 10.5958/0974-360X.2016.00324.3

ABSTRACT:
S. aureus has the ability to bind specific host matrix proteins. The S.aureus surface proteins (adhesins) that mediate the binding of these proteins iscalled as MSCRAMMs to denote their role as `microbial surface components recognizing adhesive matrix molecules'. Genes encoding SaMSCRAMMs that bind collagen, fibronectin, fibrinogen, elastin and osteopontin have been identified. Unlike the genes encoding other S. aureus MSCRAMMs, the gene encoding the collagen-binding MSCRAMM (cna) is not present in every strain of S. aureus. In this study we have taken 20 clinical isolates of S. aureus and were subjected to antibiotic sensitivity pattern followed by the detection of cna gene by PCR. We have observed total resistance to penicillin followed by other group of drugs. 10% of our strains were found to have cna gene. As we had only limited isolates from restricted samples, it is important to include more number of isolates from especially bone related infections in order to rule out the same.
KEYWORDS:
Staphylococcus aureus, CNA Gene, PCR Method.
Cite:
S.V. Rupashri, P. Gopinath. Detection of CNA Gene for the Presence of Collagen Adhesion in Clinical Strains of Staphylococcus aureus. Research J. Pharm. and Tech 2016; 9(10):1626-1628.
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