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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   9

Issue No. :  10

Year :  2016

Pages :   1623-1625

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Detection of blaTEM-1 gene for ESBL Production among Clinical isolates of Pseudomonas aeruginosa



Address:   Varshitha A1 , Dr. Gopinath P2
1BDS 2 year, Saveetha Dental College, Saveetha University, Chennai
2Senior lecturer, Department of Microbiology, Saveetha University, Chennai
*Corresponding Author
DOI No: 10.5958/0974-360X.2016.00323.1

ABSTRACT:
The occurrence of multi drug resistant P. aeruginosastrains is growing in the world, limiting the therapeutic options. Conventionally it was believed that ESBLs are derivatives of TEM and SHV parent enzymes but recently in Europe it was proved that ESBLs can be of non-TEM, non-SHV lineage. In this study we have taken 20 clinical isolates of P. aeruginosa and were subjected to antibiotic sensitivity pattern followed by the detection of blaTEM-1 gene by PCR. Higher degree of resistance to routinely used antibiotics is reported, 15% of them were found to have this gene by PCR. Proper detection of desired panel of genes is required to emphasize the resistance mechanism being operated in these pathogens for better outcome.
KEYWORDS:
Pseudomonas aeruginosa, blaTEM-1, PCR, ESBLs
Cite:
Varshitha A , Gopinath P. Detection of blaTEM-1 gene for ESBL Production among Clinical isolates of Pseudomonas aeruginosa. Research J. Pharm. and Tech 2016; 9(10):1623-1625.
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