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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   9

Issue No. :  1

Year :  2016

Pages :   27-32

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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RP-HPLC Method for Simultaneous Estimation of Albendazole and Niclosamide in Oral Suspension for Veterinary Use



Address:   Murugan S*, Upendra Janardhan CH, Niranjan Babu M
Department of Pharmaceutical Analysis, Seven Hills College of Pharmacy, Tirupathi-517561, Andhra Pradesh, India.
*Corresponding Author
DOI No: DOI: 10.5958/0974-360X.2016.00006.8

ABSTRACT:
A newer, simple, accurate and selective reversed phase high performance liquid chromatographic method for the simultaneous determination of Albendazole and Niclosamide in bulk and oral suspension for veterinary use has been developed and validated. Chromatography was performed on a 250 mm × 4.6 mm i.d., 5 µm particle, ODS Hypersil C18 column with Acetonitrile : Phosphate Buffer 50:50 (v/v) and pH is adjusted to 7.0 using sodium hydroxide solution or Ortho phosphoric acid as mobile phase at a flow rate of 1.0 mL/min. PDA detection was done at 230 nm; Albendazole and Niclosamide were eluted with retention times of 4.91 and 7.44 min, respectively. The method was validated in accordance with ICH guidelines. Validation revealed the method is specific, rapid, accurate, precise, reliable and reproducible. Calibration plots were linear over the concentration ranges 80–120 µg/mL for Albendazole and Niclosamide. Limits of detection were 0.69 and 0.61 µg/mL and limits of quantification were 2.10 and 1.84 µg/mL for Albendazole and Niclosamide, respectively. The high recovery and low coefficients of variation confirm the suitability of the method for the simultaneous determination of these two drugs in bulk and oral suspension for veterinary use.
KEYWORDS:
: High performance liquid chromatography, Albendazole, Niclosamide, oral suspension, Method validation
Cite:
Murugan S, Upendra Janardhan CH, Niranjan Babu M. RP-HPLC Method for Simultaneous Estimation of Albendazole and Niclosamide in Oral Suspension for Veterinary Use. Research J. Pharm. and Tech. 9(1): Jan., 2016; Page 27-32.
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