2nd International Conference on Fostering Interdisciplinary Research In Health Sciences (ICFIRHS) 2019 (01-May-2019)        |

Journal :   Research Journal of Pharmacy and Technology

Volume No. :   8

Issue No. :  4

Year :  2015

Pages :   732-436

ISSN Print :  0974-3618

ISSN Online :  0974-360X


Registration

Allready Registrered
Click to Login

Simultaneous Spectrophotometric Estimation of Ambroxal Hydrochloride and Salbutamol Sulphate by Second Order Derivative Method in Combined Dosage Form



Address:   Rajan V. Rele*
Central Research Laboratory, D.G. Ruparel College, Matunga, Mumbai 400016.
*Corresponding Author
DOI No: 10.5958/0974-360X.2015.00072.4

ABSTRACT:
The objective of the study was to develop a simple, accurate, precise and rapid a UV spectrophotometric i.e. second order derivative method for the determination of ambroxal hydrochloride and salbutamol sulphate in combined dosage form i.e. tablets by using distilled water as a solvent. The method was further validated by ICH guidelines. The proposed second order derivative method involves the measurement of absorbance of one drug at zero crossing point of other; hence wavelengths 221.1 nm and 236.4 nm were selected for the estimation of ambroxal hydrochloride and salbutamol sulphate respectively. The linearity of the proposed method was found in the concentration range of 1 to 10 µg /ml (r2= 0.9989) for ambroxal hydrochloride and 1 to 14 µg /ml (r2= 0.9981) for salbutamol sulphate respectively. The percentage mean recovery was found to be 100.813 % for ambroxal hydrochloride and 100.065 % for salbutamol sulphate respectively. The method was also statistically validated for its linearity, accuracy and precision. Both intra and inter day variations showed less percentage (%) RSD values indicating high grade of precision of this method.
KEYWORDS:
UV spectrophotometric estimation, second order derivative method, ambroxal hydrochloride, Salbutamol sulphate.
Cite:
Rajan V. Rele. Simultaneous Spectrophotometric Estimation of Ambroxal Hydrochloride and Salbutamol Sulphate by Second Order Derivative Method in Combined Dosage Form. Research J. Pharm. and Tech. 8(4): April, 2015; Page 432-436.
[View HTML]      [View PDF]



Visitor's No. :   644843