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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   8

Issue No. :  4

Year :  2015

Pages :   462-467

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Isolation of Flavonoids and their Anticancer Activity from Sphaeranthus amaranthoides in A549 Cell Line



Address:   Swarnalatha. Y*
Department of Biotechnology, Sathyabama University, Jeppiaar Nagar, Chennai-119.
*Corresponding Author
DOI No: 10.5958/0974-360X.2015.00077.3

ABSTRACT:
Flavonoids are considered as potential explore to treat cancer and they are ubiquitous. The chemical structure and the nature of the polyphenolic flavonoids are responsible for their anticancer actions. Objective: The present investigation concentrates on the cytotoxic effect of flavonoids in A549 adenocarcinoma cell lines. Methodology: the plant extract was subjected to TLC for confirmation and HPLC confirm the pure form of the flavonoids and the induction of the cytotoxicity was studied using AO/EB, PI and the uptake of the PI was confirmed using the FACS in A549 cells. results: the TLC and HPLC results confirmed the presence of flavonoides. Flow cytometry denotes the percentage of the cells in the G0/G1 and the G2 stage. This indicates the accumulation of the cells in the apoptotic and proapoptotic stages. Conclusion: These findings indicate that the flavonoids from the Sphaeranthus amarathoides may be promising anticancer agents to control the cell growth in A549 cells.
KEYWORDS:
Flavonoids; cancer; FACS; A549; Sphaeranthus amaranthoides.
Cite:
Swarnalatha. Y. Isolation of Flavonoids and their Anticancer Activity from Sphaeranthus amaranthoides in A549 Cell Line. Research J. Pharm. and Tech. 8(4): April, 2015; Page 462-467.
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