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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   8

Issue No. :  2

Year :  2015

Pages :   189-193

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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In vitro α-amylase and α-glucosidase inhibition and increased glucose uptake of Morinda citrifolia fruit and scopoletin



Address:   Masitah Khamis1, Fazilah Talib1, Nor Syamira Rosli1, Saravanan Dharmaraj*2, Khamsah Suryati Mohd1, Sasidharan Srenivasan3, Zubaidi Abdul Latif2, Mahadeva Rao S. Utharkar2
1Faculty of Agriculture, Biotechnology and Food Sciences, Universiti Sultan Zainal Abidin, Tembila Campus, 22200 Besut, Terengganu, Malaysia
2Faculty of Medicine, Universiti Sultan Zainal Abidin, Medical Campus, 20400 Kuala Terengganu, Terengganu, Malaysia
3Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800, Pulau Pinang, Malaysia
*Corresponding Author
DOI No: Not Available

ABSTRACT:
Diabetes mellitus is a metabolic disorder and management of blood glucose level is an important strategy in the control of the disease and complications associated with it. Therefore, components that cause uptake of glucose from the bloodstream and inhibitors of carbohydrate hydrolyzing enzymes can be useful in treatment of diabetes and medicinal plants are often used to achieve this aim. Morinda citrifolia fruit (MCF) is used in various countries for treatment of diabetes and the purpose of this study was to investigate the effect of MCF extract and its biomarker scopoletin on glucose uptake in HepG2 cells as well as its inhibitory effect on a-amylase and a-glucosidase. The safe doses for MCF extract and scopoletin were at 1 mg/ml and 0.2 µM, respectively as assessed by MTT assays and these were used for the assays. The extract had glucose uptake of 59.5% which was comparable to the standard metformin whereas the value for scopoletin was 30.6%. The extract had mild inhibitory activity on a-amylase and a-glucosidase with percentage of inhibition at 43.5% and 57%. The biomarker scopoletin showed lower activities at 23.9% and 35.7% for a-amylase and a-glucosidase respectively. Hence, these three activities may possibly be the mechanisms for MCF to exert its antidiabetic activity.
KEYWORDS:
Morinda citrifolia; scopoletin, glucose uptake, glucosidase; amylase.
Cite:
Masitah Khamis, Fazilah Talib, Nor Syamira Rosli, Saravanan Dharmaraj, Khamsah Suryati Mohd, Sasidharan Srenivasan, Zubaidi Abdul Latif, Mahadeva Rao S. Utharkar. In vitro α-amylase and α-glucosidase inhibition and increased glucose uptake of Morinda citrifolia fruit and scopoletin. Research J. Pharm. and Tech. 8(2): Feb. 2015; Page 189-193.
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