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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   8

Issue No. :  10

Year :  2015

Pages :   1399-1402

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Spectrum of Antimicrobial Susceptibility of E. coli and Staphylococcus aureus Isolates from Clinical Samples



Address:   S. Mohammed Shahid1*, Dr. Nawaz Umar2
1Lecturer, Dept. of Microbiology, Khaja Bandanawaz institute of Medical Sciences, Gulbarga- 585104, Karnataka.
2Associate Professor, Gulbarga institute of Medical Sciences, Gulbarga-585104, Karnataka
*Corresponding Author
DOI No: 10.5958/0974-360X.2015.00251.6

ABSTRACT:
Background and objectives: Resistance of antibiotics is increasing the public health threat and serious clinical problems in treatment. Aim: To determine the antibiogram of E.coli and Staphylococcus aureus isolates and to discuss sensitivity and resistance pattern. Methodology: The Kirby Bauer method was used to evaluate the susceptibility of 120 isolates each of E.coli and Staphylococcus aureus to various antibiotic drugs on Mueller-Hinton agar. Results: The prevalence of multiple drug resistance as determined on Mueller Hinton agar was 87.8% for E.coli and 90.55% for Staphylococcus aureus. For E.coli, resistance ranged from 7.5% (gentamicin and amikacin) to 89.16% (cotrimoxazole, ampicillin). In the case of Staphylococcus aureus resistance ranged from 15.83% (erythromycin and gentamicin) to 94.16 % (penicillin). Conclusion: High levels of resistance seen in this present study. And an attempt was done to find out the most resistant antibacterial drug for routine infections.
KEYWORDS:
E.coli, antimicrobial resistance, Staphylococcus aureus, antibiotic susceptibility testing, Kirby-Bauer, Muller-Hinton agar.
Cite:
S. Mohammed Shahid, Nawaz Umar. Spectrum of Antimicrobial Susceptibility of E. coli and Staphylococcus aureus Isolates from Clinical Samples. Research J. Pharm. and Tech. 8(10): Oct., 2015; Page 1399-1402.
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