2nd International Conference on Fostering Interdisciplinary Research In Health Sciences (ICFIRHS) 2019 (01-May-2019)        |

Journal :   Research Journal of Pharmacy and Technology

Volume No. :   8

Issue No. :  1

Year :  2015

Pages :   

ISSN Print :  0974-3618

ISSN Online :  0974-360X


Registration

Allready Registrered
Click to Login

Simultaneous Equation Method and Absorption Correction Method for the Estimation of Isosorbide Dinitrate and Hydalazine Hydrochloride in Bulk and Their Combined Tablet Dosage Form using UV Spectrophotometry.



Address:   Department of Pharmaceutical Analysis, University College of Pharmaceutical Sciences, Acharya Nagarjuna University, Nagarjunanagar, Guntur–522510.Andhra Pradesh (India)
DOI No: Not Available

ABSTRACT:
In the present work a simple, accurate and precise method has been developed and validated for the simultaneous estimation of isosorbide di nitrate (ISD), hydralazine hydrochloride(HYD)in bulk and their combined dosage form by UV spectrophotometric methods. The method employes estimation of drugs by simultaneous equation method (SEM) using 208.28nm and 232nm i.e λmax values of ISD and HYD respectively. Method B employs the estimation of drugs by absorption correction method (ACM) at 232nm for HYD where absorbance of ISD is very low. Absorbance correction method was based on the property of additively of absorbance. At 232nm, Hydralazine hydrochoride showed absorbance while Isosorbide dinitrate showed very low absorbance. The method involved measurement of absorbance at wavelength 232 nm. For estimation of HYD, corrected absorbance was calculated at 208 nm due to the interference of HYD at this wavelength. Calibration curves were linear with correlation coefficient 0.999 over the concentration range of 2-10μg/mL and 2-10 μg/mL for ISD and HYD respectively. The percent recoveries of the drugs were found nearly 100 % representing the accuracy of the both methods. Validation of the proposed methods was carried out for its accuracy, precision, specificity, linearity and limit of detection according to ICH guidelines. The proposed methods can be successfully applied in routine work for the determination of isosorbide dinitrate and hydralazine hydrochloride in combined dosage form.
KEYWORDS:
Isosorbide dinitrate, hydralazine hydrochloride, Absorption correction method, Simultaneous equation method.
Cite:
Not Available
[View HTML]     



Visitor's No. :   660826