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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   7

Issue No. :  8

Year :  2014

Pages :   906-909

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Simple and Rapid Simultaneous RP-HPLC Method for Determination of Glimepiride and Metformin in Tablet Dosage Form



Address:   S. M. Sandhya1*, Shiji Kumar P.S.2
1Department of Pharmaceutical Analysis, Sree Krishna College of Pharmacy & Research Centre, Parassala P.O, Thiruvananthapuram - 695502, Kerala, India.
2Department of Pharmaceutical Analysis, Jamia Salafiya Pharmacy College, Pulikkal P.O, Malappuram - 673637, Kerala, India
*Corresponding Author
DOI No: Not Available

ABSTRACT:
A simple, precise, rapid and reproducible reversed phase high performance liquid chromatography method is developed for the simultaneous estimation of glimepiride and metformin present in multicomponent dosage forms. Chromatography is carried out binary system on phenomenex C18 column (250 × 4.6 mm, 5 µm) with a mobile phase consisted of acetonitrile: acetate buffer (pH 4) in the ratio of 65:35, v/v at a flow rate of 1.0 ml/min. Detection is carried out using UV-PDA detector at 245 nm. The Rt for glimepiride and metformin are 5.709 ± 0.02 and 2.517 ± 0.05 minute respectively. The LOQ of glimepiride and metformin 0.038 and 0.221 µg/ml respectively. The RSD for three replicate measurements in three concentrations of samples in tablet are always less than 2%.
KEYWORDS:
Glimepiride, Metformin, Tablet dosage form, RP-HPLC.
Cite:
S. M. Sandhya, Shiji Kumar P.S. Simple and Rapid Simultaneous RP-HPLC Method for Determination of Glimepiride and Metformin in Tablet Dosage Form . Research J. Pharm. and Tech. 7(8): August 2014 Page 906-909.
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